DrosDel Immunity Panel
For more information about the DrosDel Immunity Panel, please check out our information page.
Shop By
Shopping Options
| Name | VDRC Id | Library | Sub-Category | Genotype | Comment | Construct Id | Publication | Provider Scientist | Provider Organization | Gene Symbol | FlyBase gene number | Synonyms | Sequence | RRID | Comment | Gene Name | Keywords | Price (net) | Add to Cart | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 349900 |
349900
|
DD-Im
|
mutants
|
w[1118]; ; Rel[E20]
|
BL1. A deletion of Relish (which also affects a nearby gene). The ebony marker of the original stock (Dan Hultmark‘s lab) was removed by recombination with an Oregon stock. The Rel[E20] mutation was then introgressed in the w[1118] DrosDel genetic background. Rel[E20] lack a functional Imd pathway and are susceptible to Gram negative bacterial infection. The transcription factor Relish may also be involved in the immune defence against viruses downstream of cGas-like, Sting and IKK (Cai et al., Curr Opin Immumol (2022)).
|
349900
|
Bruno Lemaitre with the help of Mark Hanson
|
EPFL
|
Rel
|
|
DrosDel Immunity panel
NF-KB NF-kappaB NF-κB NFkappaB NFκB Nuclear Factor-kappa-B p110 Nuclear factor NF-kappa-B p110 subunit REL RELI RELISH Rel Rel-p110 Rel/NF-kappaB Rel/NF-κB Rel1 Rel49 RelA Relish |
|
|
BL1. A deletion of Relish (which also affects a nearby gene). The ebony marker of the original stock (Dan Hultmark‘s lab) was removed by recombination with an Oregon stock. The Rel[E20] mutation was then introgressed in the w[1118] DrosDel genetic background. Rel[E20] lack a functional Imd pathway and are susceptible to Gram negative bacterial infection. The transcription factor Relish may also be involved in the immune defence against viruses downstream of cGas-like, Sting and IKK (Cai et al., Curr Opin Immumol (2022)).
|
Relish
|
DrosDel Immunity panel
|
Sign In to show prices | ||||
| 349901 |
349901
|
DD-Im
|
mutants
|
w[1118]; ; spz[rm7]/TM6C, Sb[1]
|
BL2. spz[rm7] or spz[4] is a genetically null mutation in spz (generated by EMS during Nusslein-Volhard and Wieschaus screen). Several markers of the original stock (M317 Tubingen stock center) including ebony were removed by recombination (Lemaitre et al., Cell 1996). spz[rm7] are homozygous viable, female sterile. This mutation blocks the activation of the Toll pathway. Note that the Toll pathway might be activated independently of spz by spz[5] (Nonaka et al., Biochem Biophys Res Comm 2018)).
|
349901
|
Bruno Lemaitre with the help of Mark Hanson
|
EPFL
|
spz
|
|
CT19282
DrosDel Immunity panel Protein spaetzle precursor SPZ Spaetzle Spatzle Spatzle-1 Spz Spz-1 Spz1 mel(3)7 spaetzle spatzle spazle spaztle spz |
|
|
BL2. spz[rm7] or spz[4] is a genetically null mutation in spz (generated by EMS during Nusslein-Volhard and Wieschaus screen). Several markers of the original stock (M317 Tubingen stock center) including ebony were removed by recombination (Lemaitre et al., Cell 1996). spz[rm7] are homozygous viable, female sterile. This mutation blocks the activation of the Toll pathway. Note that the Toll pathway might be activated independently of spz by spz[5] (Nonaka et al., Biochem Biophys Res Comm 2018)).
|
spatzle
|
DrosDel Immunity panel
|
Sign In to show prices | ||||
| 349902 |
349902
|
DD-Im
|
mutants
|
w[1118]; PPO1[Delta], PPO2[Delta]
|
BL3. PPO1-delta and PPO2-delta mutations were generated by homologous recombination (homologous replacement of PPO1 sequences with w[+]), isogenized in the w[1118] DrosDel genetic background. PPO1-Delta, PPO2-delta show no hemolymphatic mutation after injury. PPO3 in lamellocytes can still contribute to melanization around wasp eggs (Dudzic et al, BMC Biol 2015)
|
349902
|
Bruno Lemaitre with the help of Mark Hanson
|
EPFL
|
PPO1
|
|
A1
A[[1]] Bc Black Cell CG5779 CG8193 Diphenol oxidase A1 subunit DmPPO1 DmePPOA1 Dox-A1 DoxA1 DrosDel Immunity panel Monophenol oxidase Monophenoloxidase Mox PO PO A1 PO54 PPO PPO-1 PPO-A1 PP |
|
|
BL3. PPO1-delta and PPO2-delta mutations were generated by homologous recombination (homologous replacement of PPO1 sequences with w[+]), isogenized in the w[1118] DrosDel genetic background. PPO1-Delta, PPO2-delta show no hemolymphatic mutation after injury. PPO3 in lamellocytes can still contribute to melanization around wasp eggs (Dudzic et al, BMC Biol 2015)
|
Prophenoloxidase 1
|
DrosDel Immunity panel
|
Sign In to show prices | ||||
| 349903 |
349903
|
DD-Im
|
mutants
|
w[1118]; NimC1[Delta]; eater[Delta]
|
BL4. NimC1-delta and Eater-delta mutations were generated by homologous recombination (both genes are replaced by a w+ cassette), isogenized in the w[1118] DrosDel genetic background. NimC1-delta, Eater-delta have strongly reduced phagocytosis ability and defective hemocyte sessility due to the absence of Eater. While NimC1-delta, Eater-delta larvae have more hemocytes, NimC1-delta, Eater-delta adults tend to have decreased hemocyte number over time. This line can be used to assess the function of phagocytosis, although it likely impacts other processes.
|
349903
|
Bruno Lemaitre with the help of Mark Hanson
|
EPFL
|
NimC1
|
|
BG:DS00180.10
CG6124 CT25688 DrosDel Immunity panel Nim NimC NimC1 Nimrod C1 NimrodC1 P1 eater nimC1 nimrod nimrod C1 nimrodC1 |
|
|
BL4. NimC1-delta and Eater-delta mutations were generated by homologous recombination (both genes are replaced by a w+ cassette), isogenized in the w[1118] DrosDel genetic background. NimC1-delta, Eater-delta have strongly reduced phagocytosis ability and defective hemocyte sessility due to the absence of Eater. While NimC1-delta, Eater-delta larvae have more hemocytes, NimC1-delta, Eater-delta adults tend to have decreased hemocyte number over time. This line can be used to assess the function of phagocytosis, although it likely impacts other processes.
|
Nimrod C1
|
DrosDel Immunity panel
|
Sign In to show prices | ||||
| 349904 |
349904
|
DD-Im
|
mutants
|
w[1118], Hayan-psh[Def]
|
BL5. Hayan-psh[Def] is a short deletion generated by Shu Kondon removing 2 serine proteases, isogenized in the w[1118] DrosDel genetic background, and strongly reduces both the melanization and the Toll pathways.
|
349904
|
Bruno Lemaitre with the help of Mark Hanson
|
EPFL
|
Hayan
|
|
DrosDel Immunity panel
Hayan NimC1 Rel SP31 c-SP31 cSP31 eater |
|
|
BL5. Hayan-psh[Def] is a short deletion generated by Shu Kondon removing 2 serine proteases, isogenized in the w[1118] DrosDel genetic background, and strongly reduces both the melanization and the Toll pathways.
|
Hayan
|
DrosDel Immunity panel
|
Sign In to show prices | ||||
| 349905 |
349905
|
DD-Im
|
mutants
|
w[1118], Hayan-psh[Def]; NimC1[Delta]; eater[Delta], Rel[E20]/TM6C, Sb[1]
|
BL6. w[1118], Hayan-psh[Def]; NimC1[Delta]; Eater[Delta], Rel[E20] (also called [delta-ITPM), isogenized in the w[1118] DrosDel genetic background. Stock is viable but extremely immune deficient, having both reduced Toll pathway and Melanization (due to Hayan-psh[Def]), no phagocytosis (due to NimC1[Delta]; Eater[Delta] and no Imd pathway (Rel[E20]). This stock is maintained with a balancer on the third (TM6C). Some homozygotes present.
|
349905
|
Bruno Lemaitre with the help of Mark Hanson
|
EPFL
|
Hayan
|
|
DrosDel Immunity panel
Hayan NimC1 Rel SP31 c-SP31 cSP31 eater |
|
|
BL6. w[1118], Hayan-psh[Def]; NimC1[Delta]; Eater[Delta], Rel[E20] (also called [delta-ITPM), isogenized in the w[1118] DrosDel genetic background. Stock is viable but extremely immune deficient, having both reduced Toll pathway and Melanization (due to Hayan-psh[Def]), no phagocytosis (due to NimC1[Delta]; Eater[Delta] and no Imd pathway (Rel[E20]). This stock is maintained with a balancer on the third (TM6C). Some homozygotes present.
|
Hayan
|
DrosDel Immunity panel
|
Sign In to show prices | ||||
| 349906 |
349906
|
DD-Im
|
mutants
|
iso w[1118]
|
BL7. The DrosDel wild-type (isogenized for chr 1;2;3, with w[1118] line) used as reference. A contaminating nora virus was discovered in this stock that may have affected survival data in studies prior to 2021. Nora virus infection is associated with slightly increased mortality upon infection (anecdotally ~15%), and greatly reduced lifespan (see (15)). The DrosDel w[1118] isonneic genotype may be particularly susceptible to nora virus compared to other backgrounds (e.g. OregonR). However, nora virus-free survival and lifespan are similar to or better than OregonR.
|
349906
|
Bruno Lemaitre with the help of Mark Hanson
|
EPFL
|
w
|
|
BACN33B1.1
DMWHITE DrosDel Immunity panel EG:BACN33B1.1 Hid W c23 e(g) enhancer of garnet m(g) mini-white modifier of garnet mw w w(AT)[[13]] white white[+] |
|
|
BL7. The DrosDel wild-type (isogenized for chr 1;2;3, with w[1118] line) used as reference. A contaminating nora virus was discovered in this stock that may have affected survival data in studies prior to 2021. Nora virus infection is associated with slightly increased mortality upon infection (anecdotally ~15%), and greatly reduced lifespan (see (15)). The DrosDel w[1118] isonneic genotype may be particularly susceptible to nora virus compared to other backgrounds (e.g. OregonR). However, nora virus-free survival and lifespan are similar to or better than OregonR.
|
white
|
DrosDel Immunity panel
|
Sign In to show prices | ||||
| 349907 |
349907
|
DD-Im
|
mutants
|
w[1118]; ; Sp7[SK6]
|
BL8. Null mutation affecting the Sp7/MP2 serine protease that regulates melanization in Drosophila, isogenized in the w[1118] DrosDel genetic background. Sp7 strongly blocks the blackening reaction at the injury site of larvae but has less effect in adults (Dudzic et al., 2015).
|
349907
|
Bruno Lemaitre with the help of Mark Hanson
|
EPFL
|
Sp7
|
|
DrosDel Immunity panel
MP1 MP2 MP2/sp7/PAE1 Melanization Protease 2 PAE PAE1 SP13 SP7 Serine protease 7 Serine protease-7 Sp 7 Sp7 anon-Ryu cSP7 proPO-AE prophenoloxidase-activating enzyme prophenoloxidas |
|
|
BL8. Null mutation affecting the Sp7/MP2 serine protease that regulates melanization in Drosophila, isogenized in the w[1118] DrosDel genetic background. Sp7 strongly blocks the blackening reaction at the injury site of larvae but has less effect in adults (Dudzic et al., 2015).
|
Serine protease 7
|
DrosDel Immunity panel
|
Sign In to show prices | ||||
| 349908 |
349908
|
DD-Im
|
mutants
|
w[1118], Hayan[SK6]
|
BL9. Null mutation affecting the Hayan serine protease that regulates melanization in Drosophila, isogenized in the w[1118] DrosDel genetic background. This line was generatd by Shu Kondo with the following deletion: GGGAATCTGCCT--------------gcgagaacatcaga -14
|
349908
|
Bruno Lemaitre with the help of Mark Hanson
|
EPFL
|
Hayan
|
|
DrosDel Immunity panel
Hayan NimC1 Rel SP31 c-SP31 cSP31 eater |
|
|
BL9. Null mutation affecting the Hayan serine protease that regulates melanization in Drosophila, isogenized in the w[1118] DrosDel genetic background. This line was generatd by Shu Kondo with the following deletion: GGGAATCTGCCT--------------gcgagaacatcaga -14
|
Hayan
|
DrosDel Immunity panel
|
Sign In to show prices | ||||
| 349909 |
349909
|
DD-Im
|
mutants
|
w[1118]; ; PGRP-SD[SK1]
|
BL10. Null mutation affecting the secreted pattern recognition receptor PGRP-SD that promotes the activation of the Imd pathway upstream of PGRP-LC, isogenized in the w[1118] DrosDel genetic background.
|
349909
|
Bruno Lemaitre with the help of Mark Hanson
|
EPFL
|
PGRP-SD
|
|
Dm PGRP-SD
DrosDel Immunity panel PGRP-SD Peptidoglycan recognition protein SD Peptidoglycan-recognition protein-SD precursor pgrp-sd |
|
|
BL10. Null mutation affecting the secreted pattern recognition receptor PGRP-SD that promotes the activation of the Imd pathway upstream of PGRP-LC, isogenized in the w[1118] DrosDel genetic background.
|
Peptidoglycan recognition protein SD
|
DrosDel Immunity panel
|
Sign In to show prices | ||||
| 349910 |
349910
|
DD-Im
|
mutants
|
w[1118]; ; PGRP-LB[Delta]
|
BL11. Null mutation affecting PGRP-LB (created by homologous replacement of PGRP-LB sequences with w[+]), which encodes a secreted negative regulator of the Imd pathway upstream of PGRP-LC, isogenized in the w[1118] DrosDel genetic background.
|
349910
|
Bruno Lemaitre with the help of Mark Hanson
|
EPFL
|
PGRP-LD
|
|
CG32912
CG5523 DrosDel Immunity panel PGRP-LD Peptidoglycan recognition protein LD Peptidoglycan recognition protein-LD |
|
|
BL11. Null mutation affecting PGRP-LB (created by homologous replacement of PGRP-LB sequences with w[+]), which encodes a secreted negative regulator of the Imd pathway upstream of PGRP-LC, isogenized in the w[1118] DrosDel genetic background.
|
Peptidoglycan recognition protein LD
|
DrosDel Immunity panel
|
Sign In to show prices | ||||
| 349911 |
349911
|
DD-Im
|
mutants
|
w[1118]; Def[SK3], AttC[Mi], Dro-AttAB[SK2], Mtk[R1], Dpt[SK1]/CyO; Drs[R1], AttD[SK1]/TM6C, Sb[1]
|
BL12. DeltaAMP10: 7 deletions removing 10 Antimicrobial peptides genes, all isogenized in the w[1118] DrosDel genetic background. DroAtt removes Drosocin locus (2 peptides (Hanson et al. 2022)) and Attacin A and B. The MtkR1 and DrsR1 loci each carry one w+ transgene, and the AttCMi mutation also causes GFP to be expressed in the eyes, ocelli, and occasionally the gut. Balancers floating: CyO; TM6C, Sb (not Tubby).
|
349911
|
Bruno Lemaitre with the help of Mark Hanson
|
EPFL
|
AttA
|
|
143607_at
AHA ATT ATTA Att Att A Att-A AttA AttC AttD AttaA Attacin Attacin A Attacin-A Attacin-A precursor Attacin-A AttacinA AttacinA Attackin BcDNA:LP05763 CecA-C DEF DIM 26 Def Defe |
|
|
BL12. DeltaAMP10: 7 deletions removing 10 Antimicrobial peptides genes, all isogenized in the w[1118] DrosDel genetic background. DroAtt removes Drosocin locus (2 peptides (Hanson et al. 2022)) and Attacin A and B. The MtkR1 and DrsR1 loci each carry one w+ transgene, and the AttCMi mutation also causes GFP to be expressed in the eyes, ocelli, and occasionally the gut. Balancers floating: CyO; TM6C, Sb (not Tubby).
|
Attacin-A
|
DrosDel Immunity panel
|
Sign In to show prices |