Heidelberg CFD CRISPR Library

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Name VDRC Id Library Sub-Category Genotype Comment Construct Id Publication Provider Scientist Provider Organization Gene Symbol FlyBase gene number Synonyms HD_CFD ID HDtools ID Target sgRNA1 Target sgRNA2 Insertion Site Vector RRID Comment Name Gene Name Keywords Price (net) Add to Cart
340000
340000
HDtools
Cas9
P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=UAS-uXSCas9}attP40
for very high Cas9 levels
340000
UAS-uXSCas9
HD_CFDtools001
attP40
pUASgattB
for very high Cas9 levels
P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=UAS-uXSCas9}attP40
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340001
340001
HDtools
Cas9
P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=UAS-uSCas9}attP40
for high Cas9 levels
340001
UAS-uSCas9
HD_CFDtools002
attP40
pUASgattB
for high Cas9 levels
P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=UAS-uSCas9}attP40
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340002
340002
HDtools
Cas9
P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=UAS-uMCas9}attP40
for medium Cas9 levels
340002
UAS-uMCas9
HD_CFDtools003
attP40
pUASgattB
for medium Cas9 levels
P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=UAS-uMCas9}attP40
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340003
340003
HDtools
Cas9
P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=UAS-uLCas9}attP40
for low Cas9 levels
340003
UAS-uLCas9
HD_CFDtools004
attP40
pUASgattB
for low Cas9 levels
P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=UAS-uLCas9}attP40
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340004
340004
HDtools
Cas9
P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=UAS-uXLCas9}attP40
for very low Cas9 levels
340004
UAS-uXLCas9
HD_CFDtools005
attP40
pUASgattB
for very low Cas9 levels
P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=UAS-uXLCas9}attP40
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340005
340005
HDtools
Cas9
P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=UAS-uXXLCas9}attP40
for very low Cas9 levels, expresses mEGFP
340005
UAS-uXXLCas9
HD_CFDtools006
attP40
pUASgattB
for very low Cas9 levels, expresses mEGFP
P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=UAS-uXXLCas9}attP40
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340006
340006
HDtools
Cas9
P{ry[+t7.2]=hsFLP}12, y[1] w[*]; M{3xP3-RFP.attP=UAS-uMCas9}ZH-51D
for medium Cas9 levels
340006
UAS-uMCas9
HD_CFDtools007
ZH-51D
pUASgattB
for medium Cas9 levels
P{ry[+t7.2]=hsFLP}12, y[1] w[*]; M{3xP3-RFP.attP=UAS-uMCas9}ZH-51D
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340007
340007
HDtools
Cas9
P{ry[+t7.2]=hsFLP}12, y[1] w[*]; ;PBac{y[+]-attP-3B=UAS-uMCas9}VK00033
for medium Cas9 levels
340007
UAS-uMCas9
HD_CFDtools008
attPVK00033
pUASgattB
for medium Cas9 levels
P{ry[+t7.2]=hsFLP}12, y[1] w[*]; ;PBac{y[+]-attP-3B=UAS-uMCas9}VK00033
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340017
340017
HDtools
Gal4 Cas9
y[1] M{w[+mC]=Act5C-Cas9.P}ZH-2A w[*]; ; tub-Gal4/TM3,Ser,Sb
for ubiquitous mutagenesis
340017
act-cas9 tub-Gal4
HD_CFDtools010
ZH-2A
pAct5C
for ubiquitous mutagenesis
y[1] M{w[+mC]=Act5C-Cas9.P}ZH-2A w[*]; ; tub-Gal4/TM3,Ser,Sb
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340020
340020
HDtools
Gal4 Cas9
w[*]; PBac{y[+]-attP-3B=UAS-uMCas9}VK00033 P{Gal4}hh-Gal4/TM6B
hhGal4 UAS-uMCas9 recombined on 3rd
340020
hh-Gal4 UAS-uMCas9(VK33)
HD_CFDtools021
attPVK00033
pUASgattB
hhGal4 UAS-uMCas9 recombined on 3rd
w[*]; PBac{y[+]-attP-3B=UAS-uMCas9}VK00033 P{Gal4}hh-Gal4/TM6B
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340022
340022
HDtools
Gal4 Cas9
w[*]; P{y[+t7.7] w[+mC]=UAS-uMCas9}attP40, P{GAL4::VP16-nos.UTR}CG6325MVD1/TM6B
for germline mutagenesis
340022
nos-Gal4VP16 UAS-uMCas9(attP40)
HD_CFDtools023
attP40
pUASgattB
for germline mutagenesis
w[*]; P{y[+t7.7] w[+mC]=UAS-uMCas9}attP40, P{GAL4::VP16-nos.UTR}CG6325MVD1/TM6B
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342916
342916
HD-CFD
sgRNA
342916
Port et al., eLife, 2021
CG4820
cfd
hd
hd_cfd
heidelberg
heidelberg_cfd
HD_CFD01545
CTGTGCTGGCTCGTTGGTAG
CCGCAGGCGTGAAGATCTGA
attP40
pCFD6
heidelberg_cfd
cfd
hd
hd_cfd
heidelberg
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