J. Brennecke Stocks (JB-STOCK)
JB-STOCK
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| Name | VDRC Id | Library | Sub-Category | Genotype | Comment | Construct Id | Publication | Provider Scientist | Provider Organization | Gene Symbol | FlyBase gene number | Synonyms | Sequence | RRID | Comment | Gene Name | Keywords | Price (net) | Add to Cart | ||
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| 313488 |
313488
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JB-STOCK
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mutant allele
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w; rhi[18-7]/CyO; ;
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rhi knock-out generated via classical homologous recombination of entire Rhino CDS. Replaced by loxP flanked mini-white and attB site.
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313488
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rhi
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empty
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rhi knock-out generated via classical homologous recombination of entire Rhino CDS. Replaced by loxP flanked mini-white and attB site.
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| 313496 |
313496
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JB-STOCK
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piRNA biogenesis reporter
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w; piRNA-biogenesis-reporter #4 [attp40]/CyO; ;
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Gblock cloned into plasmid JB634 (trigger sensor plasmid version 2 from Dominik Handler); Gblock: trigger 4 (trigger: siRNA derived from white-sh hairpin); sequence: GGACGAGCTGTACAAGTAATGATCGAGTCAGATAAGCCGTATCAAAAACAAGATATGACCTATAGAAAGGAAACTACTCAAATATAACCAAAAATTGAAGCAGTCATCAAGTGAACGAAAGAAATGAAAACATGACACGTGATCAACTCGAGCGCATAGAAATTAATTTATAAATTGCAAAACCTAATTACGTAGCTAAACAAAAAACCCAAAAGAATAATGTAAGCAAAAAGAAAATTTAATCGTCATAATCGTCACCAAACCTTTTGATTGGCGAGCATTTCATAGATGTTAAATTTTCCTAATTCGAGAATCCCAGGAGGATAAGCGATAGGGATGATCAGAA
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313496
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empty
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Gblock cloned into plasmid JB634 (trigger sensor plasmid version 2 from Dominik Handler); Gblock: trigger 4 (trigger: siRNA derived from white-sh hairpin); sequence: GGACGAGCTGTACAAGTAATGATCGAGTCAGATAAGCCGTATCAAAAACAAGATATGACCTATAGAAAGGAAACTACTCAAATATAACCAAAAATTGAAGCAGTCATCAAGTGAACGAAAGAAATGAAAACATGACACGTGATCAACTCGAGCGCATAGAAATTAATTTATAAATTGCAAAACCTAATTACGTAGCTAAACAAAAAACCCAAAAGAATAATGTAAGCAAAAAGAAAATTTAATCGTCATAATCGTCACCAAACCTTTTGATTGGCGAGCATTTCATAGATGTTAAATTTTCCTAATTCGAGAATCCCAGGAGGATAAGCGATAGGGATGATCAGAA
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| 313499 |
313499
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JB-STOCK
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tethering lines
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; pUASp>LacI_GFP [attP40]/CyO; lacO_nos>GFP_Piwi (with intron) [attP2]/TM3, Ser;
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cross to germline specific Gal4 for tethering LacI_GFP to upstream of nanos promoter; based on stock 839
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313499
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empty
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cross to germline specific Gal4 for tethering LacI_GFP to upstream of nanos promoter; based on stock 839
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| 313500 |
313500
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JB-STOCK
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mutant allele
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w[1118]; CG9754[guide1_m3-4]/CyO;;
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CG9754 frameshift allele induced by Cas9 and guide RNA #1; strong allele, but genetically not as strong as those induced with guide RNA #2; mother stock: isogenic (2nd/3rd) w[1118] stock from Kirsten Senti (KS18); sequence at CG9754 locus: ACGTAGTTTTCCcccgttttCCTCTAGCTTCATCGGAGCTTCCATGATGAAAAATC
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313500
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CG9754
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empty
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CG9754 frameshift allele induced by Cas9 and guide RNA #1; strong allele, but genetically not as strong as those induced with guide RNA #2; mother stock: isogenic (2nd/3rd) w[1118] stock from Kirsten Senti (KS18); sequence at CG9754 locus: ACGTAGTTTTCCcccgttttCCTCTAGCTTCATCGGAGCTTCCATGATGAAAAATC
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| 313502 |
313502
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JB-STOCK
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mutant allele
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w[1118]; CG9754[guide2_m1-9]/CyO;;
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CG9754 frameshift allele induced by Cas9 and guide RNA #2; strong allele; genetically stronger than those induced with guide RNA #1; mother stock: isogenic (2nd/3rd) w[1118] stock from Kirsten Senti (KS18); sequence at CG9754 locus: GGACATAGGGACTCCGCAGGCAAACGCggaGTGGCGGAGTCTCGCCTGTGcCCTCC
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313502
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CG9754
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empty
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CG9754 frameshift allele induced by Cas9 and guide RNA #2; strong allele; genetically stronger than those induced with guide RNA #1; mother stock: isogenic (2nd/3rd) w[1118] stock from Kirsten Senti (KS18); sequence at CG9754 locus: GGACATAGGGACTCCGCAGGCAAACGCggaGTGGCGGAGTCTCGCCTGTGcCCTCC
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| 313510 |
313510
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JB-STOCK
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tagged construct
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w; piwi[1], BAC(GFP-Piwi[ADK]) [attP40]/CyO; ;
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piwi[1] allele with genomic rescue construct in attP40 expressing GFP-Piwi catalytic dead
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313510
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piwi
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empty
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piwi[1] allele with genomic rescue construct in attP40 expressing GFP-Piwi catalytic dead
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| 313513 |
313513
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JB-STOCK
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tagged construct
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w; piwi[1], Bac(GFP-Piwi[deltaNLS] #1) attP40/CyO; ;
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piwi[1] allele with genomic rescue construct in attP40 expressing GFP-Piwi with NLS deleted
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313513
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piwi
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empty
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piwi[1] allele with genomic rescue construct in attP40 expressing GFP-Piwi with NLS deleted
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| 313514 |
313514
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JB-STOCK
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tagged construct
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w; piwi[1], Bac(GFP-Piwi[deltaNLS] #2) attP40/CyO; ;
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piwi[1] allele with genomic rescue construct in attP40 expressing GFP-Piwi with NLS deleted
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313514
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piwi
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empty
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piwi[1] allele with genomic rescue construct in attP40 expressing GFP-Piwi with NLS deleted
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| 313521 |
313521
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JB-STOCK
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RNAi lines
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w; ; pGLKD>white_sh [attP2]/TM3, Sb ;
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in Dominik Handler's germline knockdown vector (plasmid JB227); sh-oligo sequence: ctagcagtAACAAGATATGACCTATAGAAtagttatattcaagcataTTCTATAGGTCATATCTTGTTgcg
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313521
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w
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empty
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in Dominik Handler's germline knockdown vector (plasmid JB227); sh-oligo sequence: ctagcagtAACAAGATATGACCTATAGAAtagttatattcaagcataTTCTATAGGTCATATCTTGTTgcg
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| 313523 |
313523
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JB-STOCK
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RNAi lines
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w; ;pW20>Su(var)3-9_sh1 [attP2]/TM3, Sb;
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most likely in pW20 vector, but possibly also in pGLKD vector from Dominik Handler; oligo sequence: CTAGCAGTATGCGTCGAGATGGATCAATATAGTTATATTCAAGCATATATTGATCCATCTCGACGCATGCG
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313523
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Su(var)3-9
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empty
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most likely in pW20 vector, but possibly also in pGLKD vector from Dominik Handler; oligo sequence: CTAGCAGTATGCGTCGAGATGGATCAATATAGTTATATTCAAGCATATATTGATCCATCTCGACGCATGCG
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| 313524 |
313524
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JB-STOCK
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RNAi lines
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w; ; pW20>Su(var)3-9_sh2 [attP2]/TM3, Sb;
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most likely in pW20 vector, but possibly also in pGLKD vector from Dominik Handler; oligo sequence: CTAGCAGTCAGCTCTACGAGATATACATATAGTTATATTCAAGCATATATGTATATCTCGTAGAGCTGGCG
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313524
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Su(var)3-9
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empty
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most likely in pW20 vector, but possibly also in pGLKD vector from Dominik Handler; oligo sequence: CTAGCAGTCAGCTCTACGAGATATACATATAGTTATATTCAAGCATATATGTATATCTCGTAGAGCTGGCG
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| 313527 |
313527
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JB-STOCK
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mutant alleles
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w[1118]; piwi [g1]/CyO; iso3;
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piwi Cas9 allele (guide RNA1; KS3-1m1-1); generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); stock is difficult to keep; eliminate all non-CyO flies each generation; it carries the following frameshift sequence: w1118: ATCCTCGTATAGAGGC piwi_g1:ATCCTCG−−TAGAGGC (-2)
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313527
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Piwi KS3-1m1-1
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empty
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piwi Cas9 allele (guide RNA1; KS3-1m1-1); generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); stock is difficult to keep; eliminate all non-CyO flies each generation; it carries the following frameshift sequence: w1118: ATCCTCGTATAGAGGC piwi_g1:ATCCTCG−−TAGAGGC (-2)
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