J. Brennecke Stocks (JB-STOCK)
JB-STOCK
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| Name | VDRC Id | Library | Sub-Category | Genotype | Comment | Construct Id | Publication | Provider Scientist | Provider Organization | Gene Symbol | FlyBase gene number | Synonyms | Sequence | RRID | Comment | Gene Name | Keywords | Price (net) | Add to Cart | ||
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| 313399 |
313399
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JB-STOCK
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tethering lines
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; pUASp>LacI_Piwi [attP40]/CyO; lacO_nos>GFP-Piwi (with intron) [attP2]/TM3, Ser;
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cross to germline specific Gal4 for tethering Piwi to upstream of nanos promoter, plasmid: JB516; based on stock 839
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313399
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Piwi
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empty
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cross to germline specific Gal4 for tethering Piwi to upstream of nanos promoter, plasmid: JB516; based on stock 839
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| 313400 |
313400
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JB-STOCK
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tethering lines
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; pUASp>LacI_CG9754 [attP40]/CyO; lacO_nos>GFP_Piwi (with intron) [attP2]/TM3, Ser;
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cross to germline specific Gal4 for tethering CG9754 to upstream of nanos promoter; plasmid: JB510; based on stock 839
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313400
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CG9754
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empty
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cross to germline specific Gal4 for tethering CG9754 to upstream of nanos promoter; plasmid: JB510; based on stock 839
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| 313408 |
313408
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JB-STOCK
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tethering lines
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; If/CyO; tub>EGFP_5xBoxB_SV40 [attP2]/TM3, Ser;
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GFP sensor for RNA tethering; GFP expressed under ubiquitous tubulin promoter with SV40 3'UTR where 5 boxB sites were introduced, plasmid: JB425
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313408
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empty
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GFP sensor for RNA tethering; GFP expressed under ubiquitous tubulin promoter with SV40 3'UTR where 5 boxB sites were introduced, plasmid: JB425
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| 313409 |
313409
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JB-STOCK
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tethering lines
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; pUASp>LacI_Su(var)205 [attP40]/CyO; lacO_nos>GFP_Piwi (with intron) [attP2]/TM3, Ser;
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cross to germline specific Gal4 for tethering Su(var)205 to upstream of nanos promoter, plasmid: JB512; ; based on stock 839
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313409
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Su(var)205
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empty
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cross to germline specific Gal4 for tethering Su(var)205 to upstream of nanos promoter, plasmid: JB512; ; based on stock 839
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| 313411 |
313411
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JB-STOCK
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Tagged construct
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COG-Gal4; NGT-Gal4/CyO; nosGal4, GFP_Gtsf1/TM3,Ser;
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MTD-Gal4 line that also expresses GFP-Gtsf1 from BAC-construct
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313411
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Gtsf1
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empty
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MTD-Gal4 line that also expresses GFP-Gtsf1 from BAC-construct
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| 313413 |
313413
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JB-STOCK
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RNAi lines
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; ; pGLKD>aub_sh1[attP2]/TM3;
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in Dominik Handler's germline knockdown vector (pJB227); sh-oligo sequence: CTAGCAGTCGCGACAGTGACGATTATCAATAGTTATATTCAAGCATATTGATAATCGTCACTGTCGCGGCG;
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313413
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aub
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empty
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in Dominik Handler's germline knockdown vector (pJB227); sh-oligo sequence: CTAGCAGTCGCGACAGTGACGATTATCAATAGTTATATTCAAGCATATTGATAATCGTCACTGTCGCGGCG;
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| 313431 |
313431
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JB-STOCK
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RNAi lines
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w; pGLKD>rhi_sh1 [attP40]/CyO; Sb/Tm3, Ser;
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sh-line (same construct as in stock 522): in Dominik Handler's germline knockdown vector (plasmid JB227); sh-oligo sequence: ctagcagtTACCAGATGAATGACGATCTAtagttatattcaagcataTAGATCGTCATTCATCTGGTAgcg
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313431
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rhi
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empty
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sh-line (same construct as in stock 522): in Dominik Handler's germline knockdown vector (plasmid JB227); sh-oligo sequence: ctagcagtTACCAGATGAATGACGATCTAtagttatattcaagcataTAGATCGTCATTCATCTGGTAgcg
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| 313432 |
313432
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JB-STOCK
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RNAi lines
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w; ; pGLKD>GFP_sh [attP2]/TM3, Sb ;
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in Dominik Handler's germline knockdown vector (plasmid JB227); sh-oligo sequence: ctagcagtCAAGCTGGAGTACAACTACAAtagttatattcaagcataTTGTAGTTGTACTCCAGCTTGgcg
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313432
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GFP
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empty
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in Dominik Handler's germline knockdown vector (plasmid JB227); sh-oligo sequence: ctagcagtCAAGCTGGAGTACAACTACAAtagttatattcaagcataTTGTAGTTGTACTCCAGCTTGgcg
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| 313433 |
313433
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JB-STOCK
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RNAi lines
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w; ; pGLKD>del_sh/TM3, Sb ;
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in Dominik Handler's germline knockdown vector (plasmid JB227); sh-oligo sequence: copied from TRIP line BL 32375
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313433
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del
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empty
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in Dominik Handler's germline knockdown vector (plasmid JB227); sh-oligo sequence: copied from TRIP line BL 32375
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| 313479 |
313479
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JB-STOCK
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Tagged construct
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w; ; 3xFLAG/V5/Precission/GFP(replacing CG13741 CDS)+NLS [attP2]/TM3,Sb;
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Pacman CH322-08N15 containing CG13741 CDs replaced by N-terminal GFP_Precission_V5_3xFLAG; plasmid JB625; Glycerol #783
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313479
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CG13741
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empty
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Pacman CH322-08N15 containing CG13741 CDs replaced by N-terminal GFP_Precission_V5_3xFLAG; plasmid JB625; Glycerol #783
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| 313481 |
313481
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JB-STOCK
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tagged construct
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w; ;Nxf3_GFP_Precission_V5_3xFLAG [attP2]/TM3,Sb;
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Pacman CH322-97E07 containing the Nxf3 locus with C-terminal GFP_Precission_V5_3xFLAG; plasmid JB626; Glycerol #784
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313481
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Nxf3
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empty
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Pacman CH322-97E07 containing the Nxf3 locus with C-terminal GFP_Precission_V5_3xFLAG; plasmid JB626; Glycerol #784
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| 313487 |
313487
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JB-STOCK
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mutant allele
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w; rhi[18-5]/CyO; ;
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rhi knock-out generated via classical homologous recombination of entire Rhino CDS. Replaced by loxP flanked mini-white and attB site.
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313487
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rhi
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empty
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rhi knock-out generated via classical homologous recombination of entire Rhino CDS. Replaced by loxP flanked mini-white and attB site.
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