F. Besse Stocks (FB-STOCK)

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Name VDRC Id Library Sub-Category Genotype Comment Construct Id Publication Provider Scientist Provider Organization Gene Symbol FlyBase gene number Synonyms Sequence RRID Comment Gene Name Keywords Price (net) Add to Cart
310600
310600
FB-STOCK
tagged construct
w*; me31B[KI.EGFP]
The EGFP sequence was inserted via Crispr/Cas9 in the me31B locus, at the C-terminus of the me31B coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae EGFP positive. Me31B is the Drosophila ortholog of DDX-6; it is a P-body marker.
310600
Florence Besse
Institute de Biologie Valrose (iBV) (http://ibv.unice.fr/)
me31B
C-terminal EGFP tag
DDX6
Dhh1p/Me31b
DmRH3
EGFP
ME31B
Mat31B
Maternal expression at 31B
Me31B
Me31b
P-body marker
knock-in
l(2)k06607
maternal expression at 31B
me31B
me31b
see publication
The EGFP sequence was inserted via Crispr/Cas9 in the me31B locus, at the C-terminus of the me31B coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae EGFP positive. Me31B is the Drosophila ortholog of DDX-6; it is a P-body marker.
maternal expression at 31B
knock-in
EGFP
C-terminal EGFP tag
P-body marker
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310601
310601
FB-STOCK
tagged construct
w*; me31B[KI.mTomato]
The mTomato sequence was inserted via Crispr/Cas9 in the me31B locus, at the C-terminus of the me31B coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae mTomato positive. This line can be used as a P-body marker, but not to assess granule material properties. It contains a 12 aa deletion at the end of Me31B coding sequence and the mTomato tag may affect granule material properties.
310601
Florence Besse
Institute de Biologie Valrose (iBV) (http://ibv.unice.fr/)
me31B
C-terminal mTomato tag
DDX6
Dhh1p/Me31b
DmRH3
ME31B
Mat31B
Maternal expression at 31B
Me31B
Me31b
P-body marker
knock-in
l(2)k06607
mTomato
maternal expression at 31B
me31B
me31b
see publication
The mTomato sequence was inserted via Crispr/Cas9 in the me31B locus, at the C-terminus of the me31B coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae mTomato positive. This line can be used as a P-body marker, but not to assess granule material properties. It contains a 12 aa deletion at the end of Me31B coding sequence and the mTomato tag may affect granule material properties.
maternal expression at 31B
knock-in
C-terminal mTomato tag
P-body marker
mTomato
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