310601
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SKU
FB-STOCK-310601
| VDRC Id | 310601 |
|---|---|
| Library | FB-STOCK |
| Sub-Category | tagged construct |
| Status | available |
| Comment | The mTomato sequence was inserted via Crispr/Cas9 in the me31B locus, at the C-terminus of the me31B coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae mTomato positive. This line can be used as a P-body marker, but not to assess granule material properties. It contains a 12 aa deletion at the end of Me31B coding sequence and the mTomato tag may affect granule material properties. |
| Construct Id | 310601 |
| Genotype | w*; me31B[KI.mTomato] |
| Gene Symbol | me31B |
| Gene Name | maternal expression at 31B |
| Synonyms | C-terminal mTomato tag DDX6 Dhh1p/Me31b DmRH3 ME31B Mat31B Maternal expression at 31B Me31B Me31b P-body marker knock-in l(2)k06607 mTomato maternal expression at 31B me31B me31b |
| Keywords | P-body marker knock-in mTomato C-terminal mTomato tag |
| Inserted Chromosome | 1;2 |
| Sequence | see publication |
| Viability | viable |
| Publication | Formicola et al., eLife (2021) |
| Provider Scientist | Florence Besse |
| Provider Organization | Institute de Biologie Valrose (iBV) (http://ibv.unice.fr/) |