F. Besse Stocks (FB-STOCK)
FB-STOCK
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| Name | VDRC Id | Library | Sub-Category | Genotype | Description | Construct Id | Chromosome(s) | Publication | Provider Scientist | Provider Organization | FlyBase gene number | Synonyms | Sequence | Keywords | RRID | Comment | Name | Price (net) | Add to Cart | ||
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| 310600 |
310600
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FB-STOCK
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tagged construct
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w*; me31B[KI.EGFP]
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310600
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Florence Besse
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Institute de Biologie Valrose (iBV) (http://ibv.unice.fr/)
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C-terminal EGFP tag
DDX6 Dhh1p/Me31b DmRH3 EGFP ME31B Mat31B Maternal expression at 31B Me31B Me31b P-body marker knock-in l(2)k06607 maternal expression at 31B me31B me31b |
see publication
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The EGFP sequence was inserted via Crispr/Cas9 in the me31B locus, at the C-terminus of the me31B coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae EGFP positive. Me31B is the Drosophila ortholog of DDX-6; it is a P-body marker.
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| 310601 |
310601
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FB-STOCK
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tagged construct
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w*; me31B[KI.mTomato]
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310601
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Florence Besse
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Institute de Biologie Valrose (iBV) (http://ibv.unice.fr/)
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C-terminal mTomato tag
DDX6 Dhh1p/Me31b DmRH3 ME31B Mat31B Maternal expression at 31B Me31B Me31b P-body marker knock-in l(2)k06607 mTomato maternal expression at 31B me31B me31b |
see publication
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The mTomato sequence was inserted via Crispr/Cas9 in the me31B locus, at the C-terminus of the me31B coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae mTomato positive. This line can be used as a P-body marker, but not to assess granule material properties. It contains a 12 aa deletion at the end of Me31B coding sequence and the mTomato tag may affect granule material properties.
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| 310602 |
310602
|
FB-STOCK
|
tagged construct
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w*; ; rin[KI.mCherry]
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310602
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Florence Besse
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Institute de Biologie Valrose (iBV) (http://ibv.unice.fr/)
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N-terminal mCherry tag
Next to squid Nts RIN Rasputin Rin Stress Granule marker cg9412 knock-in mCherry rasp rasputin rin |
see publication (Formicola et al., 2021; doi: 10.7554/eLife.65742.)
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The mCherry sequence was inserted via Crispr/Cas9 in the rin locus, at the N-terminus of the rin coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae mCherry. Rin is the Drosophila ortholog of the G3BP1; it is Stress Granule marker positive.
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| 310603 |
310603
|
FB-STOCK
|
tagged construct
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w*; ; Patr-1[KI.mTomato]
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310603
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Florence Besse
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Institute de Biologie Valrose (iBV) (http://ibv.unice.fr/)
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BcDNA:LD27979
C-terminal mTomato tag HPat HPat1 Hpat LD27979 P-body marker Part-1 Patr-1 Protein associated with topo II related - 1 anon-WO0118547.282 hpat knock-in l(3)06442 lethal (3) 06442 mTomato patr-1 |
see publication (Formicola et al., 2021; doi: 10.7554/eLife.65742.)
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The mTomato sequence was inserted via Crispr/Cas9 in the Patr-1 locus, at the C-terminus of the Patr-1 coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae mTomato positive. Patr-1 is the Drosophila ortholog of PATL1; it can be used as a P-body marker.
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