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Name | VDRC Id | Library | Sub-Category | Genotype | Comment | Construct Id | Publication | Provider Scientist | Provider Organization | Gene Symbol | FlyBase gene number | Synonyms | Sequence | RRID | Comment | Gene Name | Keywords | Price (net) | Add to Cart | ||
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310601 |
310601
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FB-STOCK
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tagged construct
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w*; me31B[KI.mTomato]
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The mTomato sequence was inserted via Crispr/Cas9 in the me31B locus, at the C-terminus of the me31B coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae mTomato positive. This line can be used as a P-body marker, but not to assess granule material properties. It contains a 12 aa deletion at the end of Me31B coding sequence and the mTomato tag may affect granule material properties.
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310601
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Florence Besse
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Institute de Biologie Valrose (iBV) (http://ibv.unice.fr/)
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me31B
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C-terminal mTomato tag
DDX6 Dhh1p/Me31b DmRH3 ME31B Mat31B Maternal expression at 31B Me31B Me31b P-body marker knock-in l(2)k06607 mTomato maternal expression at 31B me31B me31b |
see publication
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The mTomato sequence was inserted via Crispr/Cas9 in the me31B locus, at the C-terminus of the me31B coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae mTomato positive. This line can be used as a P-body marker, but not to assess granule material properties. It contains a 12 aa deletion at the end of Me31B coding sequence and the mTomato tag may affect granule material properties.
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maternal expression at 31B
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knock-in
C-terminal mTomato tag P-body marker mTomato |
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310800 |
310800
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AD-STOCK
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Tagged Construct
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y[1],w[1118]; P{CG10068-GFP}
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CG10068 (orthologous to human Elmod) tagged at N-terminal with GFP under Ubq promoter. Construct inserted in genome via P-element integration.
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310800
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Alexander Dammermann
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Max Perutz Labs, University of Vienna
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CG10068
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Elmod-GFP
GFP N-terminal Ubq promoter tag |
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CG10068 (orthologous to human Elmod) tagged at N-terminal with GFP under Ubq promoter. Construct inserted in genome via P-element integration.
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N-terminal
tag Elmod-GFP Ubq promoter GFP |
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310801 |
310801
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AD-STOCK
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Tagged Construct
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y[1],w[1118]; P{CG10068-GFP}
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CG10068 (orthologous to human Elmod) tagged at C-terminal with GFP under Ubq promoter. Construct inserted in genome via P-element integration.
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310801
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Alexander Dammermann
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Max Perutz Labs, University of Vienna
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CG10068
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C-terminal
Elmod-GFP GFP Ubq promoter tag |
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CG10068 (orthologous to human Elmod) tagged at C-terminal with GFP under Ubq promoter. Construct inserted in genome via P-element integration.
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C-terminal
Ubq promoter Elmod-GFP GFP tag |
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310802 |
310802
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AD-STOCK
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Tagged Construct
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y[1],w[1118]; P{nmdyn-D7-GFP}
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CG8362 (orthologous to human NME7) tagged at N-terminal with GFP under Ubq promoter. Construct inserted in genome via P-element integration.
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310802
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Alexander Dammermann
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Max Perutz Labs, University of Vienna
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nmdyn-D7
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CG10068
GFP N-terminal NME7-GFP Ubq promoter nmdyn-D7 tag |
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CG8362 (orthologous to human NME7) tagged at N-terminal with GFP under Ubq promoter. Construct inserted in genome via P-element integration.
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nmdyn-D7
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Ubq promoter
N-terminal GFP tag NME7-GFP |
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310803 |
310803
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AD-STOCK
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Tagged Construct
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y[1],w[1118]; P{nmdyn-D7-GFP}
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CG8362 (orthologous to human NME7) tagged at C-terminal with GFP under Ubq promoter. Construct inserted in genome via P-element integration.
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310803
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Alexander Dammermann
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Max Perutz Labs, University of Vienna
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nmdyn-D7
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C-terminal
CG10068 GFP NME7-GFP Ubq promoter nmdyn-D7 tag |
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CG8362 (orthologous to human NME7) tagged at C-terminal with GFP under Ubq promoter. Construct inserted in genome via P-element integration.
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nmdyn-D7
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tag
C-terminal NME7-GFP Ubq promoter GFP |
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310804 |
310804
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AD-STOCK
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Tagged Construct
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y[1],w[1118]; Rcd4-GFP
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A GFP tag has been inserted into the N-terminal end of the endogenous CG17295 (orthologous to human RCD4) coding sequence via CRISPR / gene targeting by homologous recombination.
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310804
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Alexander Dammermann
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Max Perutz Labs, University of Vienna
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Rcd4
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BcDNA:SD16838
GFP N-terminal RCD4-GFP Rcd4 Reduction in Cnn Dots 4 Reduction in Cnn dots 4 rcd4 tag |
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A GFP tag has been inserted into the N-terminal end of the endogenous CG17295 (orthologous to human RCD4) coding sequence via CRISPR / gene targeting by homologous recombination.
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Reduction in Cnn dots 4
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RCD4-GFP
GFP N-terminal tag |
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310805 |
310805
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AD-STOCK
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Tagged Construct
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y[1],w[1118]; Rcd4-mCherry
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An mCherry tag has been inserted into the N-terminal end of the endogenous CG17295 (orthologous to human RCD4) coding sequence via CRISPR / gene targeting by homologous recombination.
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310805
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Alexander Dammermann
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Max Perutz Labs, University of Vienna
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Rcd4
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BcDNA:SD16838
N-terminal RCD4-mCherry Rcd4 Reduction in Cnn Dots 4 Reduction in Cnn dots 4 mCherry rcd4 tag |
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An mCherry tag has been inserted into the N-terminal end of the endogenous CG17295 (orthologous to human RCD4) coding sequence via CRISPR / gene targeting by homologous recombination.
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Reduction in Cnn dots 4
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mCherry
RCD4-mCherry tag N-terminal |
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310806 |
310806
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AD-STOCK
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Deletion
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y[1],w[1118]; Cep97-delta
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Deletion Cep97 with ends-out method. Complete removal of CDS, clone II-6.
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310806
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Alexander Dammermann
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Max Perutz Labs, University of Vienna
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Cep97
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CT13231
Centrosomal protein 97kDa Cep97 DCep97 cep97 ends-out deletion |
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Deletion Cep97 with ends-out method. Complete removal of CDS, clone II-6.
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Centrosomal protein 97kDa
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ends-out deletion
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310807 |
310807
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AD-STOCK
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Tagged Construct
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y[1],w[1118]; P{Cep97-GFP}
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A genomic fragment (from the RP98-06H02 BAC clone) that encompasses the Cep97 transcription unit (includes 3.5kb upstream and 4.2kb downstream regulatory sequence). The coding sequence is tagged at the C-terminal end with GFP and construct inserted on chromsome 2 via P element insertion.
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310807
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Alexander Dammermann
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Max Perutz Labs, University of Vienna
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Cep97
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C-terminal
CT13231 Centrosomal protein 97kDa Cep97 Cep97-GFP DCep97 GFP cep97 tag |
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A genomic fragment (from the RP98-06H02 BAC clone) that encompasses the Cep97 transcription unit (includes 3.5kb upstream and 4.2kb downstream regulatory sequence). The coding sequence is tagged at the C-terminal end with GFP and construct inserted on chromsome 2 via P element insertion.
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Centrosomal protein 97kDa
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Cep97-GFP
GFP C-terminal tag |
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310813 |
310813
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AD-STOCK
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Deletion
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y[1],w[1118]; CG6191-delta
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CG6191 (orthologous to human CABLES1 and CABLES2) deletion created via CRISPR using 2 guide RNAs. Deletions confirmed by PCR.
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310813
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Alexander Dammermann
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Max Perutz Labs, University of Vienna
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CG6191
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BcDNA:GH06193
CABLES1 CABLES2 CRISPR Dromel_CG6191_FBtr0087678_mORF cables deletion |
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CG6191 (orthologous to human CABLES1 and CABLES2) deletion created via CRISPR using 2 guide RNAs. Deletions confirmed by PCR.
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CABLES1
CRISPR CABLES2 deletion |
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310817 |
310817
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AD-STOCK
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Tagged Construct
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y[1],w[1118]; P{CG10137-GFP}
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CG10137 (orthologous to human CEP104) tagged at C-terminal with GFP via pUBQ-vector and gateway cloning. Construct inserted in genome via P-element integration.
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310817
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Alexander Dammermann
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Max Perutz Labs, University of Vienna
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CG10137
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C-terminal
CEP104 GFP P-element insertion pUBQ vector tag |
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CG10137 (orthologous to human CEP104) tagged at C-terminal with GFP via pUBQ-vector and gateway cloning. Construct inserted in genome via P-element integration.
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tag
CEP104 P-element insertion GFP pUBQ vector C-terminal |
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310819 |
310819
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AD-STOCK
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Deletion
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y[1],w[1118]; w+-CG10137-delta
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CG10137 (orthologous to human CEP104) deletion generated by Flp‐FRT recombination between two transposon elements flanking its coding sequence (Parks et al, 2004): PBac{RB}CG10137e00472 and PBac{WH}CG10137f04546. This results in white+ eyes.
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310819
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Alexander Dammermann
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Max Perutz Labs, University of Vienna
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CG10137
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CEP104
CRISPR deletion |
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CG10137 (orthologous to human CEP104) deletion generated by Flp‐FRT recombination between two transposon elements flanking its coding sequence (Parks et al, 2004): PBac{RB}CG10137e00472 and PBac{WH}CG10137f04546. This results in white+ eyes.
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deletion
CEP104 CRISPR |
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