RNAi Toolkit Stocks (RNAi-TK)

RNAi-TK
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Name VDRC Id Library Sub-Category Genotype Comment Construct Id Publication Provider Scientist Provider Organization Gene Symbol FlyBase gene number Synonyms Left Primer Right Primer Hairpin Length Insertion Site Vector Template Sequence FlyBase Reference Reports RRID Comment Gene Name Keywords Price (net) Add to Cart
60100
60100
RNAi-TK
Landing site VIE-260B, insertion on the 2nd chromosome at position 22019296 (5` to CR33987), for more details http://stockcenter.vdrc.at/control/protocols | y,w[1118];P{attP,y[+],w[3`]
Annotated insertion: landing site VIE-260B (position chr2L: 22019296, cytological band 40D3) and a previously non-annotated insertion: (position chr2L: 9437482, cytological band 30B3). See http://www.ncbi.nlm.nih.gov/pubmed/24577271 for more details.
60100
empty
empty
genomic
empty
Annotated insertion: landing site VIE-260B (position chr2L: 22019296, cytological band 40D3) and a previously non-annotated insertion: (position chr2L: 9437482, cytological band 30B3). See http://www.ncbi.nlm.nih.gov/pubmed/24577271 for more details.
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60101
60101
RNAi-TK
40D-UAS
Control line for KK library landing site. Landing sites at 40D and 30B in KK library host strain have been separated by meiotic recombination. Line contains Gal4-responsive UAS repeats but no functional RNAi coding sequence at 40D and no transgene insertion at 30B. Enables researchers to test whether their genetic screen of interest will be affected by ectopic tiptop expression (see Vissers et al Nat. Comm. 2016. A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth. https://www.ncbi.nlm.nih.gov/pubmed/26758424).
60101
40D
40D-UAS
empty
empty
genomic
empty
Control line for KK library landing site. Landing sites at 40D and 30B in KK library host strain have been separated by meiotic recombination. Line contains Gal4-responsive UAS repeats but no functional RNAi coding sequence at 40D and no transgene insertion at 30B. Enables researchers to test whether their genetic screen of interest will be affected by ectopic tiptop expression (see Vissers et al Nat. Comm. 2016. A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth. https://www.ncbi.nlm.nih.gov/pubmed/26758424).
40D
40D-UAS
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60102
60102
RNAi-TK
Control line for KK library. UAS sequences drive expression of a 320bp fragment of mRNA for green-fluorescent protein from jellyfish (seq. X96418.1 nts 208-413) organised as an inverted repeat for RNAi. Construct inserted into KK line host strain at 40D landing site. No insertion at 30B site.
60102
CTGACTGAATTCGGACGACGGGAACTACAAGA
CTGACTGAATTCGGACGACGGGAACTACAAGA
genomic
GGACGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAGGGAGACACCCTCGTCAACAGGATCGAGCTTAAGGGAATCGATTTCAAGGAGGACGGAAACATCCTCGGCCACAAGTTGGAATACAACTACAACTCCCACAACGTATACATCATGGCCGACAAGCAAAAGAACGGCATCAAAGCCAACTTCAAGACCCGCCACAACATCGAAGACGGCGGCGTGCAACTCGCTGATCATTATCAACAAAATACTCCAATTGGCGATGACCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTT
Control line for KK library. UAS sequences drive expression of a 320bp fragment of mRNA for green-fluorescent protein from jellyfish (seq. X96418.1 nts 208-413) organised as an inverted repeat for RNAi. Construct inserted into KK line host strain at 40D landing site. No insertion at 30B site.
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60103
60103
RNAi-TK
Control line for KK library. UAS sequences drive expression of a 320bp fragment of mRNA for green-fluorescent protein from jellyfish (seq. X96418.1 nts 208-413) organised as an inverted repeat for RNAi. Construct inserted into KK line host strain at 30B landing site. No insertion at 40D site.
60103
CTGACTGAATTCGGACGACGGGAACTACAAGA
CTGACTGAATTCGGACGACGGGAACTACAAGA
genomic
GGACGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAGGGAGACACCCTCGTCAACAGGATCGAGCTTAAGGGAATCGATTTCAAGGAGGACGGAAACATCCTCGGCCACAAGTTGGAATACAACTACAACTCCCACAACGTATACATCATGGCCGACAAGCAAAAGAACGGCATCAAAGCCAACTTCAAGACCCGCCACAACATCGAAGACGGCGGCGTGCAACTCGCTGATCATTATCAACAAAATACTCCAATTGGCGATGACCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTT
Control line for KK library. UAS sequences drive expression of a 320bp fragment of mRNA for green-fluorescent protein from jellyfish (seq. X96418.1 nts 208-413) organised as an inverted repeat for RNAi. Construct inserted into KK line host strain at 30B landing site. No insertion at 40D site.
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60200
60200
RNAi-TK
w[1118];P{VDRCsh60200}attP40
Control line for shRNA stocks - contains pWALIUM20 but with no hairpin insertion, integrated into the same attP40 genetic background as the VDRC shRNA stocks.
60200
empty
empty
genomic
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Control line for shRNA stocks - contains pWALIUM20 but with no hairpin insertion, integrated into the same attP40 genetic background as the VDRC shRNA stocks.
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60201
60201
RNAi-TK
w[1118];P{VDRCsh60201}attP40
Control line for shRNA stocks - contains pWALIUM20 with a small hairpin RNA insertion under control of UAS for RNAi of EGFP and EYP, integrated into the same attP40 genetic background as the VDRC shRNA stocks.
60201
CAAGCTGGAGTACAACTACAA
TTGTAGTTGTACTCCAGCTTG
oligo
ctagcagtCAAGCTGGAGTACAACTACAAtagttatattcaagcataTTGTAGTTGTACTCCAGCTTGgcg
Control line for shRNA stocks - contains pWALIUM20 with a small hairpin RNA insertion under control of UAS for RNAi of EGFP and EYP, integrated into the same attP40 genetic background as the VDRC shRNA stocks.
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60008
60008
RNAi-TK
w[1118]; P{UAS-dicer2, w[+]}
UAS-dicer2 insertion on 2nd
60008
empty
empty
genomic
empty
UAS-dicer2 insertion on 2nd
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60010
60010
RNAi-TK
w[1118], P{UAS-dicer2, w[+]}; Pin / Cyo
UAS-dicer2 insertion on X
60010
empty
empty
genomic
empty
UAS-dicer2 insertion on X
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60013
60013
RNAi-TK
P{UAS-dicer2, w[+]}; Sp, hs-hid / Cyo
UAS-dicer2 insertion on X
60013
empty
empty
genomic
empty
UAS-dicer2 insertion on X
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