RNAi Toolkit Stocks (RNAi-TK)
RNAi-TK
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| Name | VDRC Id | Library | Sub-Category | Genotype | Construct Id | Left Primer | Right Primer | Hairpin Length | Template | Sequence | RRID | Comment | Price (net) | Add to Cart | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 60012 |
60012
|
RNAi-TK
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P{UAS-dicer2, w[+]}
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60012
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empty
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empty
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genomic
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empty
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UAS-dicer2 insertion on X
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| 60013 |
60013
|
RNAi-TK
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P{UAS-dicer2, w[+]}; Sp, hs-hid / Cyo
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60013
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empty
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empty
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genomic
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empty
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UAS-dicer2 insertion on X
|
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| 60014 |
60014
|
RNAi-TK
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P{UAS-dicer2, w[+]}; Ly, hs-hid / TM3, Sb
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60014
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empty
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empty
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genomic
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empty
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UAS-dicer2 insertion on X
|
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| 60100 |
60100
|
RNAi-TK
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Landing site VIE-260B, insertion on the 2nd chromosome at position 22019296 (5` to CR33987), for more details http://stockcenter.vdrc.at/control/protocols | y,w[1118];P{attP,y[+],w[3`]
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60100
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empty
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empty
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genomic
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empty
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Annotated insertion: landing site VIE-260B (position chr2L: 22019296, cytological band 40D3) and a previously non-annotated insertion: (position chr2L: 9437482, cytological band 30B3). See http://www.ncbi.nlm.nih.gov/pubmed/24577271 for more details.
|
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| 60101 |
60101
|
RNAi-TK
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40D-UAS
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60101
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empty
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empty
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genomic
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empty
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Control line for KK library landing site. Landing sites at 40D and 30B in KK library host strain have been separated by meiotic recombination. Line contains Gal4-responsive UAS repeats but no functional RNAi coding sequence at 40D and no transgene insertion at 30B. Enables researchers to test whether their genetic screen of interest will be affected by ectopic tiptop expression (see Vissers et al Nat. Comm. 2016. A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth. https://www.ncbi.nlm.nih.gov/pubmed/26758424).
|
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| 60102 |
60102
|
RNAi-TK
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60102
|
CTGACTGAATTCGGACGACGGGAACTACAAGA
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CTGACTGAATTCGGACGACGGGAACTACAAGA
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genomic
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GGACGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAGGGAGACACCCTCGTCAACAGGATCGAGCTTAAGGGAATCGATTTCAAGGAGGACGGAAACATCCTCGGCCACAAGTTGGAATACAACTACAACTCCCACAACGTATACATCATGGCCGACAAGCAAAAGAACGGCATCAAAGCCAACTTCAAGACCCGCCACAACATCGAAGACGGCGGCGTGCAACTCGCTGATCATTATCAACAAAATACTCCAATTGGCGATGACCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTT
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Control line for KK library. UAS sequences drive expression of a 320bp fragment of mRNA for green-fluorescent protein from jellyfish (seq. X96418.1 nts 208-413) organised as an inverted repeat for RNAi. Construct inserted into KK line host strain at 40D landing site. No insertion at 30B site.
|
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| 60103 |
60103
|
RNAi-TK
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60103
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CTGACTGAATTCGGACGACGGGAACTACAAGA
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CTGACTGAATTCGGACGACGGGAACTACAAGA
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genomic
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GGACGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAGGGAGACACCCTCGTCAACAGGATCGAGCTTAAGGGAATCGATTTCAAGGAGGACGGAAACATCCTCGGCCACAAGTTGGAATACAACTACAACTCCCACAACGTATACATCATGGCCGACAAGCAAAAGAACGGCATCAAAGCCAACTTCAAGACCCGCCACAACATCGAAGACGGCGGCGTGCAACTCGCTGATCATTATCAACAAAATACTCCAATTGGCGATGACCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTT
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Control line for KK library. UAS sequences drive expression of a 320bp fragment of mRNA for green-fluorescent protein from jellyfish (seq. X96418.1 nts 208-413) organised as an inverted repeat for RNAi. Construct inserted into KK line host strain at 30B landing site. No insertion at 40D site.
|
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| 60200 |
60200
|
RNAi-TK
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w[1118];P{VDRCsh60200}attP40
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60200
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empty
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empty
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genomic
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empty
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Control line for shRNA stocks - contains pWALIUM20 but with no hairpin insertion, integrated into the same attP40 genetic background as the VDRC shRNA stocks.
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| 60201 |
60201
|
RNAi-TK
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w[1118];P{VDRCsh60201}attP40
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60201
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CAAGCTGGAGTACAACTACAA
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TTGTAGTTGTACTCCAGCTTG
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oligo
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ctagcagtCAAGCTGGAGTACAACTACAAtagttatattcaagcataTTGTAGTTGTACTCCAGCTTGgcg
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Control line for shRNA stocks - contains pWALIUM20 with a small hairpin RNA insertion under control of UAS for RNAi of EGFP and EYP, integrated into the same attP40 genetic background as the VDRC shRNA stocks.
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