343003
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SKU
HD-CFD-12A-343003
| VDRC Id | 343003 |
|---|---|
| Library | HD-CFD-12a |
| Sub-Category | sgRNA |
| Status | available |
| Comment | Expression of multiplexed sgRNAs from a U6:3 promoter targeting gene FBgn0003444. |
| Construct Id | 343003 |
| Genotype | P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=HD12aCFD0004}attP40/CyO, P{GFP, w[-]} |
| Gene Symbol | smo |
| Gene Name | smoothened |
| FlyBase gene number | FBgn0003444 |
| Synonyms | CRISPR Cas12a HD12aCFD0004 SMO Smo Smoothene Smoothened dSmo knockout sgRNA smo smooth smoothened |
| Keywords | knockout sgRNA CRISPR Cas12a |
| CG Number (r6.01) | CG11561 |
| Sequence | sgRNA_1GTCGGAAAGAGTGTTTCGTTGCAsgRNA_2GACTGAAACACTTCGCTAATGACsgRNA_3CTGTGTCTATTTAGCTCTGGAATsgRNA_4GGATGCTCTGGAATTATCCTCCT |
| Vector | 343003 |
| Inserted Chromosome | 2 |
| Viability | viable |
| Publication | Port et al., biorxiv (2024) |
| Provider Scientist | Fillip Port and Michael Boutros |
| Provider Organization | DKFZ, Heidelberg |