302005
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SKU
VA-STOCK-302005
| Library | VA-STOCK |
|---|---|
| Construct Id | 302005 |
| Status | available |
| VDRC Id | 302005 |
| Comment | Expresses dsRNA for RNAi of polo. Expresses GFP-tagged polo with seven alanine substitutions disrupting the nuclear localization motif under UASp control. Synonymous substitutions make polo sequence resistant to RNAi by P{GD7563}. Homozygotes present., |
| Genotype | w[*]; P{w[+mC]=GD7563}v20177; P{y[+t7.7] w[+mC]=UASp-polo.NLS7A.GFP.RNAi-res}attP154/TM6B, Tb[1] |
| Sub-Category | RNAi, tagged construct |
| Publication | Kachaner et al. (2017). Coupling of Polo kinase activation to nuclear localization by a bifunctional NLS is required during mitotic entry. Nat. Commun. 8(1): 1701. |
| Sequence | empty |
| Synonyms | RNAi, tagged construct polo |
| Viability | unknown |