302002

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SKU
VA-STOCK-302002
More Information
Library VA-STOCK
Construct Id 302002
Status available
VDRC Id 302002
Comment Expresses dsRNA for RNAi of polo. Expresses GFP-tagged polo with a phosphomimetic T182D substitution under UASp control. Synonymous substitutions make polo sequence resistant to RNAi by P{GD7563}. Homozygotes present.,
Genotype w[*]; P{w[+mC]=GD7563}v20177; P{y[+t7.7] w[+mC]=UASp-polo.T182D.GFP.RNAi-res}attP154/TM6B, Tb[1]
Sub-Category RNAi, tagged construct
Publication Kachaner et al. (2017). Coupling of Polo kinase activation to nuclear localization by a bifunctional NLS is required during mitotic entry.  Nat. Commun. 8(1): 1701.
Sequence empty
Synonyms RNAi, tagged construct
polo
Viability unknown