100724
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SKU
KK-100724
| VDRC Id | 100724 |
|---|---|
| Library | KK |
| Status | available |
| Construct Id | 108114 |
| CG Number (r6.01) | CG11518 |
| FlyBase gene number | FBgn0028951 FBgn0039853 FBgn0043900 |
| Synonyms | BEST:LD21971 Dm Pygo Pygo Pygopus anon-WO02077023.1 dPygo gam gammy legs pNB5.2 pyg pygo pygopous pygopus pypo |
| Inserted Chromosome | 2 |
| Viability | viable |
| Hairpin Length | 425 |
| ON Targets | 1 |
| OFF Targets | 4 |
| s19 | 0.99 |
| CAN Repeats | 5 |
| Insertion @ 40D | N |
| Insertion @ 30B | Y |
| Left Primer PCR1 | tggcgcccctagatgGGGTGGTTCATCATATTCGG |
| Left Primer PCR2 | GCGCGAATTCTGGCGCCCCTAGATG(ETU) |
| Right Primer PCR1 | taggtctagccccgcTCGGTATCATCGGTAGCCTC |
| Right Primer PCR2 | GCGCTCTAGATAGGTCTAGCCCCGC(XTS2) |
| Sequence Verification Primer | ETU |
| Template | genomic |
| Sequence | GGGTGGTTCATCATATTCGGATGCGGGGGACCAAGATGGGAGGGTTGCTGCGGTGGCAGCATTTGGTTCATGCCCATTCCGGGCATTGGTCCGGGCCCGTGGCCAGGTGGGCCGCCGCCCATGTGCTGCTGGTGCTGCATAGGAACTGCGTTCGGGATCATCTGGCCCATCATGGGTCCGTTGTTGTTATTGCTGTTGTTGTTATTGGGGCCATTGGGTCCGGCTGGTGACTGCAGCGGGCTGGGTCCCGAGTTGGGCATGCCCGGACTCGGGCCGGCGCTGTTTAGCGAGTGATGCGGTGACATGGCGGGCGGCGCTGGCGAGGATGTAGGCACTGACGTGGACATGGACGTGGCCGTCGATGTGGGCGTGAGCGTGGGCGTGGCGGGCGAGGGAACCGATGAGGAGGCTACCGATGATACCGA |