100267
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SKU
KK-100267
| VDRC Id | 100267 |
|---|---|
| Library | KK |
| Status | available |
| Comment | wing mutation |
| Construct Id | 107344 |
| CG Number (r6.01) | CG5231 |
| FlyBase gene number | FBgn0029158 FBgn0036989 |
| Synonyms | Las Lipoic acid synthase |
| Inserted Chromosome | 2 |
| Viability | viable |
| Hairpin Length | 300 |
| ON Targets | 1 |
| OFF Targets | 1 |
| CAN Repeats | 3 |
| Insertion @ 40D | N |
| Insertion @ 30B | Y |
| Left Primer PCR1 | tggcgcccctagatgATAATCGTGGCTGTCTGGGT |
| Left Primer PCR2 | GCGCGAATTCTGGCGCCCCTAGATG(ETU) |
| Right Primer PCR1 | taggtctagccccgcGGAGATTCGTGAACGTCTGG |
| Right Primer PCR2 | GCGCTCTAGATAGGTCTAGCCCCGC(XTS2) |
| Sequence Verification Primer | ETU |
| Template | genomic |
| Sequence | ATAATCGTGGCTGTCTGGGTGCCATGCTCGCCGCCGCCCCAGCACTCGCCGATGTTGGGACACCGGGCCTCCTCGCAGACGGTGGACAGCTTGAGCTCGCGCATCTGGGCCTTGATTTTGGCGTAGTTTTTGCCCACGGGAATGGTGGTCTTTAACCAAGGCGGCAATCGCAGTCGCTGCTCCTCGCCCTTTTCGCGGCGTAGCTTTCCATCGTACTGTTCCCACTCGTTTCTGGTGTTGTCCGGATTCTGCACAAAGTCATGGAAATTCGGACCCTTTGCCAGACGTTCACGAATCTCC |