100146
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SKU
KK-100146
| VDRC Id | 100146 |
|---|---|
| Library | KK |
| Status | available |
| Construct Id | 105034 |
| CG Number (r6.01) | CG30049 |
| FlyBase gene number | FBgn0050049 |
| Synonyms | CG30049-PA |
| Inserted Chromosome | 2 |
| Viability | viable |
| Hairpin Length | 382 |
| ON Targets | 1 |
| OFF Targets | 1 |
| s19 | 0.99 |
| CAN Repeats | 2 |
| Insertion @ 40D | Y |
| Insertion @ 30B | Y |
| Left Primer PCR1 | tggcgcccctagatgCCGTAAACCTGACCGGACTA |
| Left Primer PCR2 | GCGCGAATTCTGGCGCCCCTAGATG(ETU) |
| Right Primer PCR1 | tagcctccctagcgcATGTGGAATTTGAATGGGGA |
| Right Primer PCR2 | GCGCTCTAGATAGCCTCCCTAGCGC(XTS4) |
| Sequence Verification Primer | ETU |
| Template | genomic |
| Sequence | CCGTAAACCTGACCGGACTAACATCAGTGGATGGATACTGCGATAGGTACGTTATGTGTGGAATACCTTGCTTTTGGAGCAGCTGGTGTAGGGGGATCTCCAGTGCTGCTTGGCTACCTCGCGATGAGGAGGTGACAGTGCCGGGAAACCTGGAACTGAACTTTCTTAATAAAACACTCTCGAAATCGGGAAGTTCTGCAAGATTCGAGTTTGAATTGGCTGGTCCACCGCACATGGGCCTGTATATAAGACCGCTGGATGGAGTAGCTGTGGAGGATTGGTCCTTTATACGAACGATGCTGGACCAGCCAGAAAAATACTCACCGCCCTATCAAATATACTTCTCTTACGGCGTGGATAGCTCCCCATTCAAATTCCACAT |