100019
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SKU
KK-100019
| VDRC Id | 100019 |
|---|---|
| Library | KK |
| Status | available |
| Construct Id | 102950 |
| CG Number (r6.01) | CG14181 |
| FlyBase gene number | FBgn 35965 FBgn0035965 |
| Synonyms | USE1 ortholog (S. cerevisiae) Unconventional SNARE in the ER 1 ortholog (S. cerevisiae) Use1 |
| Inserted Chromosome | 2 |
| Viability | viable |
| Hairpin Length | 330 |
| ON Targets | 1 |
| OFF Targets | 0 |
| CAN Repeats | 2 |
| Insertion @ 40D | N |
| Insertion @ 30B | Y |
| Left Primer PCR1 | tggcgcccctagatgGTGGCAATTACGAAGGCAAT |
| Left Primer PCR2 | GCGCGAATTCTGGCGCCCCTAGATG(ETU) |
| Right Primer PCR1 | tagcctccctagcgcACGTCGAGGAGCAGATGAAT |
| Right Primer PCR2 | GCGCTCTAGATAGCCTCCCTAGCGC(XTS4) |
| Sequence Verification Primer | ETU |
| Template | genomic |
| Sequence | GTGGCAATTACGAAGGCAATCATCAGCCACAGCCAGCACTTGTAGGCTTTTTTCGAATGTTGCTCCAGTTTCTCAGCCTCTTTTCCTAGCGAATTGATATTGCGATCCGCCATTCCGGCGGAACGCGACACAACCTCCGTGTCTCTTCGGATAATCCTGTTGGCGGTCTCCGTCTGTTCCTTTAAGTTCCGAGTCAGCGAGAGCATGTGCTCGGTGATCTTCTCCTGGGCATTCGTATAATACTTGGCAGCCTGGTTCATGTTTTCACCAGAAGTTTCCTGAACGTTTGCGCTTCCAGAACTGGAGGAGGATTCATCTGCTCCTCGACGT |