J. Brennecke Stocks (JB-STOCK)
JB-STOCK
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| Name | VDRC Id | Library | Sub-Category | Genotype | Comment | Construct Id | Publication | Provider Scientist | Provider Organization | Gene Symbol | FlyBase gene number | Synonyms | Sequence | RRID | Comment | Gene Name | Keywords | Price (net) | Add to Cart | ||
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| 313396 |
313396
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JB-STOCK
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tethering lines
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; pUASp>LacI_Mael [attP40]/CyO; lacO_nos>GFP-Piwi (with intron) [attP2]/TM3, Ser;
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cross to germline specific Gal4 for tethering Mael to upstream of nanos promoter, plasmid: JB511; based on stock 839
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313396
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Mael
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empty
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cross to germline specific Gal4 for tethering Mael to upstream of nanos promoter, plasmid: JB511; based on stock 839
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| 313528 |
313528
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JB-STOCK
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mutant alleles
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w[1118]; piwi [g2.1]/CyO; iso3;
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piwi Cas9 allele (guide RNA2; KS3-2m4-1) generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); stock is difficult to keep; eliminate all non-CyO flies each generation; this stock replaces the published strain piwi [g2], which died; it carries the following frameshift sequence: w1118: GAAGCCTCTATACGAGG piwi_g2.1:GAAGCCTCT−−CGAGG (-2)
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313528
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Piwi KS3-2m4-1
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empty
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piwi Cas9 allele (guide RNA2; KS3-2m4-1) generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); stock is difficult to keep; eliminate all non-CyO flies each generation; this stock replaces the published strain piwi [g2], which died; it carries the following frameshift sequence: w1118: GAAGCCTCTATACGAGG piwi_g2.1:GAAGCCTCT−−CGAGG (-2)
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| 313739 |
313739
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JB-STOCK
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mutant allele
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w; CG13741_A1-1/CyO;
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313739
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pan3a-m1-1; indel: 7; seq: CTTGCAAACCTACAtcccacatatcatccacatcGGGTGGCGATgaCGATGTTGAAGTTGAA
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CG13741
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empty
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| 313530 |
313530
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JB-STOCK
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mutant alleles
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w[1118]; aub [g2]/CyO; iso3;
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aub Cas9 allele (guide RNA2; KS2-2m1-1); generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); it carries the following frameshift sequence: w1118: AGGGATCCTGGGCGGC, aub_g2:AGGG−−−−−−−−CGGC (-8)
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313530
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Aub KS2-2m1-1
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empty
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aub Cas9 allele (guide RNA2; KS2-2m1-1); generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); it carries the following frameshift sequence: w1118: AGGGATCCTGGGCGGC, aub_g2:AGGG−−−−−−−−CGGC (-8)
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| 313598 |
313598
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JB-STOCK
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Crispr/Cas9
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w[1118]; iso2; Ly/TM3, Sb;
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w1118 iso1.1 with isogenized 2nd chrs and balanced on 3nd
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313598
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empty
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w1118 iso1.1 with isogenized 2nd chrs and balanced on 3nd
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| 313670 |
313670
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JB-STOCK
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GAL4 driver
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Cog-GAL4; internalGT-GAL4/CyO; nos-GAL4, spnE-GFP/TM3,Ser;
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313670
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spindle-E
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empty
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| 313671 |
313671
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JB-STOCK
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GAL4 driver
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Cog-GAL4; internalGT-GAL4/CyO; nos-GAL4, vas-GFP/TM3,Ser;
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313671
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vasa
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empty
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| 313774 |
313774
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JB-STOCK
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Tagged construct
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w, moon_FS2∆28/FM7c; pNASA > LAP-TRF2s[attP40]/CyO; pNASA > Deadlock-vhhGFP[attP2]/TM3,Ser
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complete bypass stock
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313774
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Andersen et al., Nature 2017
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moonshiner
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empty
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complete bypass stock
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| 313394 |
313394
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JB-STOCK
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tethering lines
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; If/CyO; lacO_nos>GFP-Piwi (with intron) [attP2]/TM3, Ser;
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nanos-promoter driven GFP-Piwi (including piwi introns); robust expression in germline and soma; 30nt before the nos promoter fragment 8 LacO sites are inserted; plasmid: JB507
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313394
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empty
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nanos-promoter driven GFP-Piwi (including piwi introns); robust expression in germline and soma; 30nt before the nos promoter fragment 8 LacO sites are inserted; plasmid: JB507
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