J. Brennecke Stocks (JB-STOCK)
JB-STOCK
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| Name | VDRC Id | Library | Sub-Category | Genotype | Comment | Construct Id | Publication | Provider Scientist | Provider Organization | Gene Symbol | FlyBase gene number | Synonyms | Sequence | RRID | Comment | Gene Name | Keywords | Price (net) | Add to Cart | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 314063 |
314063
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JB-STOCK
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tagged construct
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w; ; CG2678RMCE[deltaZnF#4-S161-3xFLAG]/TM3, Sb
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Tagged Kipferl construct (RMCE); 3xFLAG tagged ZnF4 deletion construct inserted into 'LB1-RMCEm31'. Aka: L50. Homozygous females subfertile.
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314063
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Julius Brennecke
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Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)
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kipf
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3x
B FLAG Kipf RMCE S161 ZnF anon-84Eb kipf kipferl tag transcript B |
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Tagged Kipferl construct (RMCE); 3xFLAG tagged ZnF4 deletion construct inserted into 'LB1-RMCEm31'. Aka: L50. Homozygous females subfertile.
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kipferl
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tag
3x FLAG S161 ZnF RMCE |
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| 314064 |
314064
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JB-STOCK
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tagged construct
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w; pRhino>3xFLAG/V5/Precission/GFP-Rhino(CSD::GCN4)[attP40], rhi[g3m13]/CyO
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Tagged Rhino construct; Rhino indel –14; Rhino CSD was replaced by the Gcn4 dimerization domain; aka: L03. Homozygous females subfertile.
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314064
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Julius Brennecke
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Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)
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rhi
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3x
B CG2678 CG32135 CSD FLAG GCN4 GFP HP1 HP1D HP1d Heterochromatin protein 1 Kipf NXF3 Nuclear export factor 3 Nxf3 Precission RHI RHINO Rhi Rhino V5 anon-84Eb g3m13 heterochromatin |
TGGGCGTCCCCAGG---------------AGCGGTTTTCCGAACGAGAACAACACC
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Tagged Rhino construct; Rhino indel –14; Rhino CSD was replaced by the Gcn4 dimerization domain; aka: L03. Homozygous females subfertile.
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rhino
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FLAG
V5 tag CSD pRhino Precission g3m13 3x GCN4 GFP |
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| 314065 |
314065
|
JB-STOCK
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tagged construct
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w; pRhino>3xFLAG/V5/Precission/GFP-Rhino(art.hinge)[attP40], rhi[g3m13]/CyO
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Tagged Rhino construct; Rhino indel –14; Rhino hinge was replaced by a scrambled amino acid sequence of the same length; aka: L17. Homozygous females subfertile.
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314065
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Julius Brennecke
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Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)
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rhi
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3x
B CG2678 CG32135 FLAG GFP HP1 HP1D HP1d Heterochromatin protein 1 Kipf NXF3 Nuclear export factor 3 Nxf3 Precission RHI RHINO Rhi Rhino V5 anon-84Eb heterochromatin protein 1 hp1d kip |
TGGGCGTCCCCAGG---------------AGCGGTTTTCCGAACGAGAACAACACC
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Tagged Rhino construct; Rhino indel –14; Rhino hinge was replaced by a scrambled amino acid sequence of the same length; aka: L17. Homozygous females subfertile.
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rhino
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GFP
tag Precission 3x FLAG pRhino V5 |
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| 314066 |
314066
|
JB-STOCK
|
tagged construct
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w; ; pRhino>3xFLAG/V5/Precission/GFP-Su(var)2-5[attP2]
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Tagged HP1a construct; aka: L48
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314066
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Julius Brennecke
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Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)
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Su(var)205
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3x
C1A9-nuclear-antigen CBX5 DmHP-1 DmHP1 E(var)29 E(var)29A FLAG GFP HP-1 HP1 HP1-VS HP1A HP1a HP1alpha Heterochromatic Protein 1 Heterochromatic Protein 1a Heterochromatin Protein 1 Heterochromatin P |
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Tagged HP1a construct; aka: L48
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Suppressor of variegation 205
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Precission
art 3x hinge pRhino V5 g3m13 tag FLAG GFP |
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| 314067 |
314067
|
JB-STOCK
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tagged construct
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w, 3xFLAG/V5/Precission/GFP-HP1b
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Endogenously tagged HP1b; aka: LB3-f212.
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314067
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Julius Brennecke
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Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)
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HP1b
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3x
FLAG GFP HP1 HP1B HP1b Heterochromatin Protein 1B Heterochromatin Protein 1b Hp1b Precission V5 hp1b tag |
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Endogenously tagged HP1b; aka: LB3-f212.
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Heterochromatin Protein 1b
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3x
GFP tag FLAG Precission V5 |
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| 314068 |
314068
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JB-STOCK
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tagged construct
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w; ; 3xFLAG/V5/Precission/GFP-HP1c
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Endogenously tagged HP1c; aka: LB4-f212.
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314068
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Julius Brennecke
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Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)
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HP1c
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3x
FLAG GFP HP1 HP1C HP1c Heterochromatin Protein 1c Heterochromatin protein 1c Hp1c Precission V5 heterochromatin protein 1c hp1c tag |
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Endogenously tagged HP1c; aka: LB4-f212.
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Heterochromatin Protein 1c
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FLAG
Precission tag 3x GFP V5 |
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| 314069 |
314069
|
JB-STOCK
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tagged construct
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w; peggless>TurboID-linker-vhhGFP-3xHA-NLS[attP40]/CyO
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TurboID biotin ligase fused to GFP nanobody, expressed from eggless regulatory elements. Aka: CY59.
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314069
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Julius Brennecke
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Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)
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GFP
GFP nanobody HA NLS TurboID biotin eggless fusion construct ligase nanobody synthetic construct vhh |
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TurboID biotin ligase fused to GFP nanobody, expressed from eggless regulatory elements. Aka: CY59.
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NLS
ligase nanobody HA GFP eggless vhh fusion construct synthetic construct TurboID GFP nanobody biotin |
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| 313801 |
313801
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JB-STOCK
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tagged construct
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w; ; 3xFLAG_V5_Precission_GFP_smt3 [attP2]/TM3,Sb;
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Pacman CH322-158C23 containing the smt3 locus with N-terminal 3xFLAG_V5_Precission_GFP; Glycerol #915
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313801
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SUMO
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empty
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Pacman CH322-158C23 containing the smt3 locus with N-terminal 3xFLAG_V5_Precission_GFP; Glycerol #915
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| 313494 |
313494
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JB-STOCK
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piRNA biogenesis reporter
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w; piRNA-biogenesis-reporter #2 [attp40]/CyO; ;
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Gblock cloned into plasmid JB634 (trigger sensor plasmid version 2 from Dominik Handler); Gblock: trigger 2 (trigger piRNA: Aub dominant); sequence: GGACGAGCTGTACAAGTAATGATCGAGTCAGATAAGCCGTATCAATCCTTCTCTCAAATTTAATTATCTAAAGGAAACTACTCAAATATAACCAAAAATTGAAGCAGTCATCAAGTGAACGAAAGAAATGAAAACATGACACGTGATCAACTCGAGCGCATAGAAATTAATTTATAAATTGCAAAACCTAATTACGTAGCTAAACAAAAAACCCAAAAGAATAATGTAAGCAAAAAGAAAATTTAATCGTCATAATCGTCACCAAACCTTTTGATTGGCGAGCATTTCATAGATGTTAAATTTTCCTAATTCGAGAATCCCAGGAGGATAAGCGATAGGGATGATCAGAA
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313494
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empty
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Gblock cloned into plasmid JB634 (trigger sensor plasmid version 2 from Dominik Handler); Gblock: trigger 2 (trigger piRNA: Aub dominant); sequence: GGACGAGCTGTACAAGTAATGATCGAGTCAGATAAGCCGTATCAATCCTTCTCTCAAATTTAATTATCTAAAGGAAACTACTCAAATATAACCAAAAATTGAAGCAGTCATCAAGTGAACGAAAGAAATGAAAACATGACACGTGATCAACTCGAGCGCATAGAAATTAATTTATAAATTGCAAAACCTAATTACGTAGCTAAACAAAAAACCCAAAAGAATAATGTAAGCAAAAAGAAAATTTAATCGTCATAATCGTCACCAAACCTTTTGATTGGCGAGCATTTCATAGATGTTAAATTTTCCTAATTCGAGAATCCCAGGAGGATAAGCGATAGGGATGATCAGAA
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| 313174 |
313174
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JB-STOCK
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RNAi lines
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w; ; pGLKD>BoYb_sh1(attP2)/TM3,Sb;
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in Dominik Handler's germline knockdown vector (plasmid JB227); sh-oligo sequence: ctagcagtCAGCTGGAAGATGAAAGTAAAtagttatattcaagcataTTTACTTTCATCTTCCAGCTGgcg; used by Rippei
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313174
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BoYb
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empty
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in Dominik Handler's germline knockdown vector (plasmid JB227); sh-oligo sequence: ctagcagtCAGCTGGAAGATGAAAGTAAAtagttatattcaagcataTTTACTTTCATCTTCCAGCTGgcg; used by Rippei
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| 313396 |
313396
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JB-STOCK
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tethering lines
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; pUASp>LacI_Mael [attP40]/CyO; lacO_nos>GFP-Piwi (with intron) [attP2]/TM3, Ser;
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cross to germline specific Gal4 for tethering Mael to upstream of nanos promoter, plasmid: JB511; based on stock 839
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313396
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Mael
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empty
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cross to germline specific Gal4 for tethering Mael to upstream of nanos promoter, plasmid: JB511; based on stock 839
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| 313739 |
313739
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JB-STOCK
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mutant allele
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w; CG13741_A1-1/CyO;
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313739
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pan3a-m1-1; indel: 7; seq: CTTGCAAACCTACAtcccacatatcatccacatcGGGTGGCGATgaCGATGTTGAAGTTGAA
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CG13741
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empty
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