J. Brennecke Stocks (JB-STOCK)
JB-STOCK
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| Name | VDRC Id | Library | Sub-Category | Genotype | Comment | Construct Id | Publication | Provider Scientist | Provider Organization | Gene Symbol | FlyBase gene number | Synonyms | Sequence | RRID | Comment | Gene Name | Keywords | Price (net) | Add to Cart | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 313695 |
313695
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JB-STOCK
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shmiR line
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w; If/CyO; pW20>zuc_sh+nbr_sh[attP2]/TM3,Ser;
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attP2 landing site, 1st zuc_shmiR sequence is identical to GL00111, 2nd nibbler_shmiR sequence is identical to nbr_sh2, 1st top primer(zucchini): CTAGCAGTTTGTTGTGCATCAAGTTCGTGTAGTTATATTCAAGCATACACGAACTTGATGCACAACAAGCG, 2nd top primer(nibbler): ccggtAGTATGGTCAGTGATCTCAGTGTATAGTTATATTCAAGCATATACACTGAGATCACTGACCATGCgcatg
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313695
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Hayashi & Schnabl, Nature, 2016
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zucchini, nibbler
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empty
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attP2 landing site, 1st zuc_shmiR sequence is identical to GL00111, 2nd nibbler_shmiR sequence is identical to nbr_sh2, 1st top primer(zucchini): CTAGCAGTTTGTTGTGCATCAAGTTCGTGTAGTTATATTCAAGCATACACGAACTTGATGCACAACAAGCG, 2nd top primer(nibbler): ccggtAGTATGGTCAGTGATCTCAGTGTATAGTTATATTCAAGCATATACACTGAGATCACTGACCATGCgcatg
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| 313700 |
313700
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JB-STOCK
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CRISPR line
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w; nibbler[Cas9 FS allele]/CyO-GFP; ;
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313700
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Hayashi & Schnabl, Nature, 2016
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nibbler
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empty
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| 313703 |
313703
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JB-STOCK
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CRISPR line
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w; FLAG-nibbler[wt]/CyO; ;
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endogenous Nibbler allele with N-terminal 3xFLAG tag
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313703
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Hayashi & Schnabl, Nature, 2016
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nibbler
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empty
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endogenous Nibbler allele with N-terminal 3xFLAG tag
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| 313705 |
313705
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JB-STOCK
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CRISPR line
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w; papi[1-5-2]; ;
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papi null allele (frameshift induced by Cas9); sequence: GACATTAGCGGCGTTCCCGccgaca-TAAGGCGGCTCGAGCTCTGCTTATCAA
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313705
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Hayashi & Schnabl, Nature, 2016
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papi
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empty
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papi null allele (frameshift induced by Cas9); sequence: GACATTAGCGGCGTTCCCGccgaca-TAAGGCGGCTCGAGCTCTGCTTATCAA
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| 313706 |
313706
|
JB-STOCK
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CRISPR line
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w; papi[4-1-1]/CyO; ;
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papi null allele (frameshift induced by Cas9); sequence: AGCGGACTCAGGTGGCCAACGGC----ATCCGGGATTAGAGGACAGACAGAGGAACAGAAGCCCCAGAAGG
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313706
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Hayashi & Schnabl, Nature, 2016
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papi
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empty
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papi null allele (frameshift induced by Cas9); sequence: AGCGGACTCAGGTGGCCAACGGC----ATCCGGGATTAGAGGACAGACAGAGGAACAGAAGCCCCAGAAGG
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| 313713 |
313713
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JB-STOCK
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Pacman recombineering
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w; If/CyO; 3xFLAG_V5_Precission_GFP_Nbr[attP2]/TM3, Ser;
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Pacman CH322-18I04 containing the Nbr locus with N-terminal 3xFLAG_V5_Precission_GFP; plasmid JB677; Glycerol #807
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313713
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Hayashi & Schnabl, Nature, 2016
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nibbler
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empty
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Pacman CH322-18I04 containing the Nbr locus with N-terminal 3xFLAG_V5_Precission_GFP; plasmid JB677; Glycerol #807
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| 313715 |
313715
|
JB-STOCK
|
Pacman recombineering
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w; Papi_GFP_Precission_V5_3xFLAG[attP40]/CyO; Sb/TM3, Ser;
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Pacman CH322-41G09 containing the papi locus with C-terminal GFP_Precission_V5_3xFLAG;plasmid JB569; Glycerol #735
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313715
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Hayashi & Schnabl, Nature, 2016
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papi
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empty
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Pacman CH322-41G09 containing the papi locus with C-terminal GFP_Precission_V5_3xFLAG;plasmid JB569; Glycerol #735
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| 313717 |
313717
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JB-STOCK
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tagged construct
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w; ; Nxt1_GFP_Precission_V5_3xFLAG [attP2]/TM3,Sb;
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Pacman CH322-123E19 containing the Nxt1 locus with C-terminal GFP_Precission_V5_3xFLAG; Glycerol #836
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313717
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p15, DmNXT2
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empty
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Pacman CH322-123E19 containing the Nxt1 locus with C-terminal GFP_Precission_V5_3xFLAG; Glycerol #836
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| 313722 |
313722
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JB-STOCK
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reporter
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UAS Dcr-2(X),hs-hid(Y); NGT-Gal4-VP16; nos-GAL4, nos>GFP_lacZ_HeT-A_500/Ser;
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germline HeT-A sensor; based on stock 537;target sequence shorter than the published one (500 nt), 2 hours instead of o/n staining
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313722
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HeT-A
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empty
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germline HeT-A sensor; based on stock 537;target sequence shorter than the published one (500 nt), 2 hours instead of o/n staining
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| 313723 |
313723
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JB-STOCK
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reporter
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UAS Dcr-2(X),hs-hid(Y); NGT-Gal4-VP16; nos-GAL4, nos>GFP_lacZ_HeT-A_700/Ser;
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germline HeT-A sensor; based on stock 537; target sequence shorter than the published one (700 nt), 2 hours instead of o/n staining
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313723
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HeT-A
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empty
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germline HeT-A sensor; based on stock 537; target sequence shorter than the published one (700 nt), 2 hours instead of o/n staining
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| 313725 |
313725
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JB-STOCK
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mutant allele
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w; cluster38C1∆LEFTprom[1-6]/CyO;
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seq: GACTCCTGCAAGCAATGAGCGATACCCCA---AGAAGAGCACTAGTAAATGAAGTGGAGACCAGGGT
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313725
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Andersen et al., Nature 2017
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Leftmost piRNA cluster at chromosomal band 38C
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empty
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seq: GACTCCTGCAAGCAATGAGCGATACCCCA---AGAAGAGCACTAGTAAATGAAGTGGAGACCAGGGT
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| 313726 |
313726
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JB-STOCK
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mutant allele
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w, moon_FS2∆28/FM7c; cluster38C1∆LEFTprom[1-6]/CyO;
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seq: GACTCCTGCAAGCAATGAGCGATACCCCA---AGAAGAGCACTAGTAAATGAAGTGGAGACCAGGGT
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313726
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Andersen et al., Nature 2017
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Leftmost piRNA cluster at chromosomal band 38C
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empty
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seq: GACTCCTGCAAGCAATGAGCGATACCCCA---AGAAGAGCACTAGTAAATGAAGTGGAGACCAGGGT
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