111003
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SKU
KK-111003
| VDRC Id | 111003 |
|---|---|
| Library | KK |
| Status | temporarily unavailable |
| Comment | KK RNAi line similar to VDRC ID 105838, but with insertion of UAS-RNAi construct in landing site at 30B only. No insertion at 40D. (See Vissers et al Nat. Comm. 2015. A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth. https://www.ncbi.nlm.nih.gov/pubmed/26758424). Stock donated by Kieran Harvey. |
| Construct Id | 102930 |
| CG Number (r6.01) | CG4236 |
| FlyBase gene number | FBgn0015610 FBgn0016688 FBgn0025477 |
| Synonyms | 154659_at 55 CAF-1 CAF1 CAF1-55 CAF1-p55 CAF1p55 Caf-1 Caf1 Caf1/p55 Caf1p55 Chromatin Assembly Factor 1 Chromatin Assembly Factor-1 Chromatin assembly factor 1 subunit Chromatin associated factor-1 subunit MSI |
| Inserted Chromosome | 2 |
| Viability | viable |
| Hairpin Length | 436 |
| ON Targets | 1 |
| OFF Targets | 0 |
| CAN Repeats | 4 |
| Insertion @ 40D | N |
| Insertion @ 30B | Y |
| Left Primer PCR1 | tggcgcccctagatgGCTTGGCATCTGCTACATGA |
| Left Primer PCR2 | GCGCGAATTCTGGCGCCCCTAGATG(ETU) |
| Right Primer PCR1 | taggtctagccccgcCGCTAATCTTGGCAGTGTGA |
| Right Primer PCR2 | GCGCTCTAGATAGGTCTAGCCCCGC(XTS2) |
| Sequence Verification Primer | ETU |
| Template | genomic |
| Sequence | GCTTGGCATCTGCTACATGAGTCTCTGTTCGGATCGGTGGCTGACGACCAGAAGCTGATGATCTGGGACACGCGCAACAACAACACCTCGAAGCCCTCGCATACAGTGGACGCTCACACAGCGGAAGTCAATTGCTTGAGCTTTAATCCCTACTCGGAGTTCATTCTGGCCACGGGCTCCGCTGACAAGACTGTAGCTCTATGGGATCTGCGCAACCTCAAGCTAAAACTACATTCCTTCGAGTCGCACAAGGACGAGATCTTCCAAGTGCAGTGGTCGCCGCATAACGAGACTATTCTCGCCTCCTCCGGCACTGACCGCCGCCTACACGTCTGGGATTTGTCCAAAATCGGCGAGGAGCAGAGTACGGAGGATGCTGAGGACGGACCACCCGAGCTGCTCTTCATCCATGGCGGTCACACTGCCAAGATTAGCG |
| Publication | Vissers et al Nat. Comm. 2016. A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth. |