110728
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SKU
KK-110728
| VDRC Id | 110728 |
|---|---|
| Library | KK |
| Status | available |
| Comment | verified manually |
| Construct Id | 102603 |
| CG Number (r6.01) | CG2863 |
| FlyBase gene number | FBgn0021874 FBgn0042663 |
| Synonyms | Nle Notchless anon-WO0026364 l(2)k13714 notchless |
| Inserted Chromosome | 2 |
| Viability | viable |
| Hairpin Length | 455 |
| ON Targets | 1 |
| OFF Targets | 2 |
| s19 | 0.99 |
| CAN Repeats | 2 |
| Insertion @ 40D | N |
| Insertion @ 30B | Y |
| Left Primer PCR1 | tggcgcccctagatgAGGATACGCACAGAACCCAC |
| Left Primer PCR2 | GCGCGAATTCTGGCGCCCCTAGATG(ETU) |
| Right Primer PCR1 | cgacgcccgctgataCACGCCACATACGATACAGG |
| Right Primer PCR2 | GCGCTCTAGACGACGCCCGCTGATA(XTS1) |
| Sequence Verification Primer | ETU |
| Template | genomic |
| Sequence | AGGATACGCACAGAACCCACTGCTTATGACCTGTGCAGGTGAAGTGCGGTGTCTCTGTGTTAAGATCCCACAATCGCACTGTGGTGTCGCCACTTCCACTGGCGAGATGAGCACCATCCGGGCTGAAATTCAGCGAAACCACAGCCTCGGCGTGTCCCGGCATGGAACTCGTGCATCTTGTCACTGGGCGCACTTTGAAAACCGCCTGTGGCTGATACACAATATCGATCACGTTTTCGGTGTCCACTGACGCCAAGTCCAACGTGTCCTCCAGGCTCTTCTTGATCTCATCCTCGCCCACGAAAAACAAATATGGAGTGGCTTCCTCGTTTTTCAGCAGCGCGTTGCAAATCAGACCCAATTGCTGGGTAGTGATTCCTGCCGGCAGGTCGATTGGCGGGCCCGCTTCCTCGCCCGTGTCCGAAACGAGGCGCGCCTGTATCGTATGTGGCGTG |