110724
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SKU
KK-110724
| VDRC Id | 110724 |
|---|---|
| Library | KK |
| Status | available |
| Comment | verified manually |
| Construct Id | 102524 |
| CG Number (r6.01) | CG3284 |
| FlyBase gene number | FBgn0004855 |
| Synonyms | H5 Pol II PolII RNA Pol II RNA pol II RNA polII RNA polymerase II RNA polymerase II 15kD subunit RNA polymerase II 15kd subunit RNAP RNAP II RNApolII RPII15 RpII15 RpII215 Rpb9 dRPB9 l(3)88Be l(3)Z23 |
| Inserted Chromosome | 2 |
| Viability | viable |
| Hairpin Length | 339 |
| ON Targets | 1 |
| OFF Targets | 0 |
| CAN Repeats | 2 |
| Insertion @ 40D | N |
| Insertion @ 30B | Y |
| Left Primer PCR1 | tggcgcccctagatgACATGCTGTACCCCAAGGAG |
| Left Primer PCR2 | GCGCGAATTCTGGCGCCCCTAGATG(ETU) |
| Right Primer PCR1 | cgcatgtagcctgccTTAGAGGCAGATGGGTCGTC |
| Right Primer PCR2 | GCGCTCTAGACGCATGTAGCCTGCC(XTS3) |
| Sequence Verification Primer | ETU |
| Template | genomic |
| Sequence | ACATGCTGTACCCCAAGGAGGACAAGGAGAACAAGATCCTGCTGTACGCCTGCCGGAATTGCGATTACAAACAGGAGGCGGACTCCAACTGCATCTACGTGAACAAGATTATGCACGAGATCGACGAGCTGACCCACATTGTGCCCGACGTGATTTCCGATCCCACGCTGCCGCGCACCGAAGACCACGCTTGTCCCAAGTGCTCCCATCGGGAGGCGGTCTTCTTCCAGGCGCAAACTCGTCGCGCCGAAGAGGAGATGCGACTGTACTACGTGTGCACCAACCAGAACTGCACCCACCGTTGGACGGAGTAGAAACTGACGACCCATCTGCCTCTAA |