110577
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SKU
KK-110577
| VDRC Id | 110577 |
|---|---|
| Library | KK |
| Status | available |
| Comment | HmZ weak |
| Construct Id | 100719 |
| CG Number (r6.01) | CG9635 |
| FlyBase gene number | FBgn0010569 FBgn0020519 FBgn0023172 |
| Synonyms | DRhoGEF DRhoGEF2 DrhoGEF2 Gef2 RG2 Rho guanine nucleotide exchange factor 2 Rho-GEF2 RhoGEF RhoGEF2 RhoGF2 RhoGef2 Shar pei Su(Rho1)2B T2 Transcription unit 2 akkordion dRhoGEF dRhoGEF2 guanine nucleot |
| Inserted Chromosome | 2 |
| Viability | sterile |
| Hairpin Length | 394 |
| ON Targets | 1 |
| OFF Targets | 2 |
| s19 | 0.99 |
| CAN Repeats | 0 |
| Insertion @ 40D | Y |
| Insertion @ 30B | Y |
| Left Primer PCR1 | tggcgcccctagatgCAGTCGAACGATACGCAGAA |
| Left Primer PCR2 | GCGCGAATTCTGGCGCCCCTAGATG(ETU) |
| Right Primer PCR1 | cgacgcccgctgataTTCGGCTACGCAGAAAAACT |
| Right Primer PCR2 | GCGCTCTAGACGACGCCCGCTGATA(XTS1) |
| Sequence Verification Primer | ETU |
| Template | genomic |
| Sequence | CAGTCGAACGATACGCAGAAGGAGATCATCTCGATGGAGGACGAAAACTCGGACTTGGATGAGCCCTTCATTGACGAGAACGGACCCTTTAACAATCTAACTCGTTTGTTAGAGGCCGAGAACGTCACTTTCCTAGCCATCTTCCTAAACTACGTGATCTCAAACTCGGATCCCGCGCCACTTCTGTTTTACCTGATTACTGAGTTGTACAAGGAGGGCACCTCCAAGGACATGCGGAAATGGGCCTACGAAATCCACTCCACATTCCTCGTGCCGCGGGCTCCATTGTCATGGTATCGCCAAGATGAATCGCTGGCCCGCGAGGTGGATAATGTCCTGCAGTTGGAGTATGACAAAGTGGAGATCCTAAGGACAGTTTTTCTGCGTAGCCGAA |