110424
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SKU
KK-110424
| VDRC Id | 110424 |
|---|---|
| Library | KK |
| Status | available |
| Comment | verified manually |
| Construct Id | 101636 |
| CG Number (r6.01) | CG4451 |
| FlyBase gene number | FBgn0038754 FBgn0038755 FBgn0038759 |
| Synonyms | 6-O sulfotransferase 6-O-sulfotransferase CG11683 CG17188 CG18533 DmHs6st Heparan sulfate 6-O-sulfotransferase Hs6st dHS6ST heparan sulfate 6-O-sulfotransferase hs6st |
| Inserted Chromosome | 2 |
| Viability | viable |
| Hairpin Length | 534 |
| ON Targets | 1 |
| OFF Targets | 1 |
| CAN Repeats | 3 |
| Insertion @ 40D | N |
| Insertion @ 30B | Y |
| Left Primer PCR1 | tggcgcccctagatgGGCGGTGTAGTAGTGTGGGT |
| Left Primer PCR2 | GCGCAGATCTTGGCGCCCCTAGATG(GTU) |
| Right Primer PCR1 | cgcatgtagcctgccACGGAATTCAACTGGGACAG |
| Right Primer PCR2 | GCGCTCTAGACGCATGTAGCCTGCC(XTS3) |
| Sequence Verification Primer | GTU |
| Template | genomic |
| Sequence | GGCGGTGTAGTAGTGTGGGTGCGTTGTGAAGGTGAAAAGTGAATTCGAGTGGGCTCGCACCTCACAATGTTTGAAAAGGGAACTTGCGTTTAAGAAATAAACATTTTATGCAAAGTATTTACATTTTCTAAAATTTTCGGTGCGCGACTTAAGCAAGGAGTATAGTTATAGTCATAGATTTTATAGTTTTGCTTAGGTTTAGGTTTAGAGATAATATACGCTCTGAGATTTTGCTCGTTTGTCCTTTAGTTGTGTTACGTTCGATTGAAACAAGCCAAGTCCGCTAGATGTTTTTTGTCTGCTTAATTAACATTATGCTTAATCGCTAGTTTAAAGTCCTTCGGTTGATTTTTATGCTGATGCTTCTGCGTATCGGTAACCTTAGTGACCACCCCCCCATATTCCGCCCTTCCCAGCTACATATTTATGAGTTGGTTTGTCCTACATCTAGTTCCTAATCGGTAATATTTGGTTTCGAGTTCAATGATCCGTGCTGAGCCCATCCTCCACGTTGCTGTCCCAGTTGAATTCCGT |