110421
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SKU
KK-110421
| VDRC Id | 110421 |
|---|---|
| Library | KK |
| Status | available |
| Comment | HmZ weak, verified manually |
| Construct Id | 101588 |
| CG Number (r6.01) | CG4973 |
| FlyBase gene number | FBgn0038772 |
| Synonyms | mdlc midlife crisis |
| Inserted Chromosome | 2 |
| Viability | sterile |
| Hairpin Length | 539 |
| ON Targets | 1 |
| OFF Targets | 1 |
| CAN Repeats | 4 |
| Insertion @ 40D | N |
| Insertion @ 30B | Y |
| Left Primer PCR1 | tggcgcccctagatgGAGCACCAAGACAATGAGCA |
| Left Primer PCR2 | GCGCGAATTCTGGCGCCCCTAGATG(ETU) |
| Right Primer PCR1 | cgcatgtagcctgccCGGAGTCTACATCACCCGTT |
| Right Primer PCR2 | GCGCTCTAGACGCATGTAGCCTGCC(XTS3) |
| Sequence Verification Primer | ETU |
| Template | genomic |
| Sequence | GAGCACCAAGACAATGAGCAAAGCAAAGAGACAGGCGGGAGGAGTTGGTGATGATGGCTCATCCTCAGCCTCCGACGATGATAAACTGGGCATTGCGTACAAATCCAAACGCGAGGCGCTGCCCAGTGGACCCCAAGATCAGGGAGCTACATCGATTAACGAAATGGACACAGAATTGGATCGCGATGCGCAGGCAATTCATGCGCGAGCTCTGAAAATCAACGAAGAGCTGGAGGGCAAGGCGGACGATAAGATCTATCGCGGAATTAACAACTATGCGCAGTACTACAAGAAGCAGGATACAGCGGCAGGGAATGCCAGCTCCGGAATGGTACGAAGTGGCCCAATTAGGGCTCCTGCCCATCTTAGGGCCACTGTCAGGTGGGATTATCAGCCGGATATATGCAAGGATTACAAGGAAACAGGATATTGCGGCTTTGGCGACAGCTGTAAGTTTCTGCACGATCGCAGTGATTACAAAGCGGGCTGGCAATTGGAAATGGATCACGAAAATCAGCGAACGGGTGATGTAGACTCCG |