110409
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SKU
KK-110409
| VDRC Id | 110409 |
|---|---|
| Library | KK |
| Status | available |
| Comment | verified manually |
| Construct Id | 101447 |
| CG Number (r6.01) | CG5247 |
| FlyBase gene number | FBgn0004048 FBgn0011292 FBgn0011774 |
| Synonyms | DmKu70 IRBP IRBP/Ku70 Inverted repeat-binding protein Irbp Ku Ku70 YPF1 YPF1beta Yolk protein factor 1b Ypf1b dp70 irbp ku70 p70 |
| Inserted Chromosome | 2 |
| Viability | viable |
| Hairpin Length | 554 |
| ON Targets | 1 |
| OFF Targets | 2 |
| s19 | 0.99 |
| CAN Repeats | 3 |
| Insertion @ 40D | Y |
| Insertion @ 30B | Y |
| Left Primer PCR1 | tggcgcccctagatgCCCGCCTTCTTGTGAAATTA |
| Left Primer PCR2 | GCGCAGATCTTGGCGCCCCTAGATG(GTU) |
| Right Primer PCR1 | taggtctagccccgcGCGTTGTTTAGCTTCTTGCC |
| Right Primer PCR2 | GCGCTCTAGATAGGTCTAGCCCCGC(XTS2) |
| Sequence Verification Primer | GTU |
| Template | genomic |
| Sequence | CCCGCCTTCTTGTGAAATTAGCGGAAAGTCAGATTTTTAGCAATCTAAAGAAGCCGCATAATGAGCACCTGGAATCCGGAGAACGATGTGGACCTGCTGTCTGGGTCCGAGGACGAGGAGGATGTGTCCATGAAGCGGGACTACCATGGGCGCGAGGCCATTCTGTTCGTGGTAGACGCCAATCTTCAGACAGCCGGCGTGGAGCGCCTGTTGGAGGCACTGAACATCATCCGGACGGCCTTTATATCCGGACTTCTGGTTAACGACAAGGACCTCATCGGACTCATCTTCGCCAACACCAAGCACAGTCCGCCGCCGCTGGAAGCCAGTGCATTGGACAACATCGTAATGCCGGATAACTGCGCAGTGTTCTTGCCCCTTCGCCAACTAACCAAACCCATTGTGGAGCACTATCTGGAATTCATGGGCGGGGTGGAGACGCAGTTCGCCGATGTGTATGGCCTGGCGGAACCCGATGGTCGCGGCAGGTTTGACCTTATGATCCGGCTCTGCATCGAGATGCTGGAAAAGTGCGGCAAGAAGCTAAACAACGC |