110216
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SKU
KK-110216
| VDRC Id | 110216 |
|---|---|
| Library | KK |
| Status | available |
| Comment | verified manually |
| Construct Id | 102675 |
| CG Number (r6.01) | CG3180 |
| FlyBase gene number | FBgn0003276 FBgn0083157 FBgn0262955 |
| Synonyms | 150kDa polypeptide DmRP140 H5 II Pol II Pol IIc PolII PolIIc RNA Pol II RNA Pol II 140 RNA Pol II second largest subunit RNA Pol IIc RNA pol II RNA pol II second largest subunit RNA polII RNA polymerase II R |
| Inserted Chromosome | 2 |
| Viability | viable |
| Hairpin Length | 451 |
| ON Targets | 1 |
| OFF Targets | 1 |
| CAN Repeats | 3 |
| Insertion @ 40D | N |
| Insertion @ 30B | Y |
| Left Primer PCR1 | tggcgcccctagatgGTTCCATGGAGTACCTGCGT |
| Left Primer PCR2 | GCGCAGATCTTGGCGCCCCTAGATG(GTU) |
| Right Primer PCR1 | cgacgcccgctgataTTGAGCGTAAGCATCACCTG |
| Right Primer PCR2 | GCGCTCTAGACGACGCCCGCTGATA(XTS1) |
| Sequence Verification Primer | GTU |
| Template | genomic |
| Sequence | GTTCCATGGAGTACCTGCGTTTCCGAGAACTACCAGCTGGCATCAATTCAATCGTGGCTATTCTCTGCTACACTGGCTACAACCAGGAGGATTCTGTCATTCTGAATGCATCCGCTGTGGAGCGTGGATTCTTCCGCTCTGTGTTCTACCGATCATACAAGGATTCGGAGAACAAACGTGTCGGTGATCAGGAAGAGAACTTCGAGAAGCCTCATCGCGGCACCTGCCAAGGAATGAGAAATGCTCACTACGACAAGTTAGACGATGATGGAATTATTGCCCCAGGAATTCGTGTATCTGGCGACGATGTGGTTATTGGCAAGACCATTACCCTTCCTGAAAACGACGACGAACTGGACAGCAACACGAAGCGTTTCTCCAAACGAGATGCCTCCACTTTCCTGCGAAACTCTGAGACGGGTATTGTGGACCAGGTGATGCTTACGCTCAA |