109454
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SKU
KK-109454
| VDRC Id | 109454 |
|---|---|
| Library | KK |
| Status | available |
| Comment | duplicate 901H12 |
| Construct Id | 108916 |
| CG Number (r6.01) | CG4637 |
| FlyBase gene number | FBgn0000159 FBgn0001191 FBgn0002748 |
| Synonyms | HH Hedgehog Hedghog Hh Mir Mirabile Moonrat Mrt anon-WO0134654.19 anon-WO0182946.19 bar bar-3 bar-on-3 bar3 hedgehog hg hh l(3)hh l(3)neo56 l(3)neo57 |
| Inserted Chromosome | 2 |
| Viability | viable |
| Hairpin Length | 336 |
| ON Targets | 1 |
| OFF Targets | 4 |
| s19 | 0.99 |
| CAN Repeats | 3 |
| Insertion @ 40D | Y |
| Insertion @ 30B | N |
| Left Primer PCR1 | tggcgcccctagatgTGACCTTGTAGAGCGCATTG |
| Left Primer PCR2 | GCGCGAATTCTGGCGCCCCTAGATG(ETU) |
| Right Primer PCR1 | tagcctccctagcgcGCATCGAGGAGAAGAACCAG |
| Right Primer PCR2 | GCGCTCTAGATAGCCTCCCTAGCGC(XTS4) |
| Sequence Verification Primer | ETU |
| Template | genomic |
| Sequence | TGACCTTGTAGAGCGCATTGGCATACCAATGGATGCCATTCTGCTGCTGCGCCGAGCTCACCACCTTCGGCGAACTGTGCAACTGCTCCTTGGCGGGCAGCCACGCCTCCAGCGTGGACAGCAGGCGCATGGGAGCCAGTCCCCAGTGGGCCAGCGACTGGCTGTTGATCACCGCATAGCAACTGGCGGCCACCGAGTTGACCACAATGGTGCCCTCGCGGGTCAGCGGCGCGACCACGCCCTTACTGCGCACACTGCCCACCTTGACGACTCGCTGGGGCCTCAGCTCGCCCGTCTCCACATCCCGTACGAGCACCTGGTTCTTCTCCTCGATGC |