105124
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SKU
KK-105124
| VDRC Id | 105124 |
|---|---|
| Library | KK |
| Status | available |
| Comment | duplicate 900D5 |
| Construct Id | 101474 |
| CG Number (r6.01) | CG12770 |
| FlyBase gene number | FBgn0021814 FBgn0033254 |
| Synonyms | DmVps28 Dvps28 ESCRT-I ESCRT-II Vacuolar protein sorting 28 Vps28 dVps28 dvps28 l(2)k16503 vps28 |
| Inserted Chromosome | 2 |
| Viability | lethal |
| Hairpin Length | 366 |
| ON Targets | 1 |
| OFF Targets | 0 |
| CAN Repeats | 3 |
| Insertion @ 40D | Y |
| Insertion @ 30B | Y |
| Left Primer PCR1 | tggcgcccctagatgCTTCTCCACCTTCAGCTTGG |
| Left Primer PCR2 | GCGCAGATCTTGGCGCCCCTAGATG(GTU) |
| Right Primer PCR1 | cgcatgtagcctgccATTACGCCGCAGGAATACAC |
| Right Primer PCR2 | GCGCTCTAGACGCATGTAGCCTGCC(XTS3) |
| Sequence Verification Primer | GTU |
| Template | genomic |
| Sequence | CTTCTCCACCTTCAGCTTGGCGTCGAAGTCCTCGGGAATGAGGGACAGGCGATTCATGTTGTCAGCCAGGTCTTTGACGTCCGGCTGTAGGGCGTCCATGGTGTTGATCTGCAGGCGTAGCTTGTCCATGATTGTGATGAACAGCGACACGATCTCGGCGATGCATTTGGAGGTGTTGCCCTTATCGTCGCGTATGGTGATGGGTCGGTCCTCGCGGATCCGCTCCAGCGCAGCAGGGCAGTCCAGCCGGAATTTCTTAACGAACGTCTCCACCGAGGGGAATTCGTCGCACTGCACCTGCTTAAAGGCCACCTTGTACTGCACCAGGTATTTGGAGCAGGCGGCCGTGTATTCCTGCGGCGTAAT |