100224
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SKU
KK-100224
| VDRC Id | 100224 |
|---|---|
| Library | KK |
| Status | available |
| Construct Id | 107162 |
| CG Number (r6.01) | CG6830 |
| FlyBase gene number | FBgn0037934 |
| Inserted Chromosome | 2 |
| Viability | lethal |
| Hairpin Length | 414 |
| ON Targets | 1 |
| OFF Targets | 1 |
| s19 | 0.99 |
| CAN Repeats | 3 |
| Insertion @ 40D | N |
| Insertion @ 30B | Y |
| Left Primer PCR1 | tggcgcccctagatgTAACCCCATCAGCAGAGTCC |
| Left Primer PCR2 | GCGCGAATTCTGGCGCCCCTAGATG(ETU) |
| Right Primer PCR1 | taggtctagccccgcATCCCAACGAGTTTAATGCG |
| Right Primer PCR2 | GCGCTCTAGATAGGTCTAGCCCCGC(XTS2) |
| Sequence Verification Primer | ETU |
| Template | genomic |
| Sequence | TAACCCCATCAGCAGAGTCCGACATAAAATTATCGAATGTGGCCGACTGAGTGGGGTCCAAGAGAACCAATGGCAGCACGGAGATGGTTGGGAATAGCACCCATCCGCCGTATTTGATGAGCGTTCGGTGCAATTCCCTCAGCGATGGCTTTCGGCCAGTGAATCCCAAAATGCCCAAGTGCTTAACCAGTGCTTCTTGGTAATGCCTGATGAAAAAGTCAAAATGGCTCAGCTTGTAATCAATTTGAACCGAGGAAATGATGAAGTAGAGCAGATCCATGGCAGGAGAGCCGTATTTGGGATTCTGGAAATCGACAAAGACCATATCCTCCAAGTCCCCGCTGGAGTTCATCTTGAACAGAAGATTATTCATCCAGCAATCGCCATGGTTTAGCGCATTAAACTCGTTGGGAT |