311062
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SKU
ML-STOCK-311062
| Library | ML-STOCK |
|---|---|
| Construct Id | 311062 |
| Status | available |
| VDRC Id | 311062 |
| Comment | Two tag arrays have been inserted into the endogenous twi locus. 32 repeats of the SunTag(v4) peptide have been inserted at the N-terminal end of the coding sequence (this allows visualization of the protein). 128 copies of the Tag:MS2 stem loop have been inserted at the N-terminal end of the 3'UTR (this allows visualization of the transcript). dsRED screening marker is positioned after twist 3'UTR, homozygote not viable |
| Name | y1,w*; twist-32x_Suntag 128x_MS2 {dsRed +}/CyO |
| Genotype | y1,w*; twist-32x_Suntag 128x_MS2 {dsRed +}/CyO |
| Sub-Category | tagged construct |
| Chromosome(s) | -1 |
| Description | Two tag arrays have been inserted into the endogenous twi locus. 32 repeats of the SunTag(v4) peptide have been inserted at the N-terminal end of the coding sequence (this allows visualization of the protein). 128 copies of the Tag:MS2 stem loop have been |
| Publication | Dufourt et al., Science (2021) |
| Sequence | empty |
| FlyBase gene number | FBgn0003900 |
| Synonyms | suntag, twi_suntag, MS2, dsRed tagged construct twist |
| Viability | unknown |