111000
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SKU
KK-111000
| VDRC Id | 111000 |
|---|---|
| Library | KK |
| Status | available |
| Comment | KK RNAi line similar to VDRC ID 107151, but with insertion of UAS-RNAi construct in landing site at 30B only. No insertion at 40D. (See Vissers et al Nat. Comm. 2015. A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth. https://www.ncbi.nlm.nih.gov/pubmed/26758424). Stock donated by Kieran Harvey. |
| Construct Id | 100936 |
| CG Number (r6.01) | CG8208 |
| FlyBase gene number | FBgn0027950 FBgn0029156 |
| Synonyms | Dm Mbd2/3 DmMbd2/3 MBD-like MBD2-3 MBD2/3 MBD3 dMBD-like dMBD2/3 dMbD2/3 methyl-CpG-binding-domain-like-protein |
| Inserted Chromosome | 2 |
| Viability | viable |
| Hairpin Length | 384 |
| ON Targets | 1 |
| OFF Targets | 0 |
| CAN Repeats | 0 |
| Insertion @ 40D | N |
| Insertion @ 30B | Y |
| Left Primer PCR1 | tggcgcccctagatgGACTGCCTCGATCTTCAAGC |
| Left Primer PCR2 | GCGCGAATTCTGGCGCCCCTAGATG(ETU) |
| Right Primer PCR1 | taggtctagccccgcCGTATGTCGTCCTCCGAAAT |
| Right Primer PCR2 | GCGCTCTAGATAGGTCTAGCCCCGC(XTS2) |
| Sequence Verification Primer | ETU |
| Template | genomic |
| Sequence | GACTGCCTCGATCTTCAAGCAACCGGTGACGGTGATTCGCAACCATAAACAGGATCCCGCCAAAGCGAAGAATGAACCCAAGCATGGCACTCGGGAGAAGCCGAAACAGCTGTTCTGGGAGAAGCGACTGGAACGACTACGCGCCTGCCACGACAGTGGCGAGGAGCTAGACGACATCTCACTGCCCAAGACCATACGCACCGTTGGCCCCAATGTCAACGAACAGACCGTCCTCCAGTCGGTGGCCACGGCCCTGCATATGCTCAACGCCGGCGTACACGGCCAGAGTTCTACGAAGGCTGATTTGACCAAGAATGCCATGGCGTTTATGAATCCTGAACAACCGCTAATGCACGCGGTGATCATTTCGGAGGACGACATACG |
| Publication | Vissers et al Nat. Comm. 2016. A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth. |