100523

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SKU
KK-100523
More Information
VDRC Id 100523
Library KK
Status available
Construct Id 104749
CG Number (r6.01) CG9720
FlyBase gene number FBgn0039783
Synonyms PH4alphaNE2
prolyl-4-hydroxylase-alpha NE2
Inserted Chromosome 2
Viability viable
Hairpin Length 350
ON Targets 1
OFF Targets 0
CAN Repeats 3
Insertion @ 40D Y
Insertion @ 30B Y
Left Primer PCR1 tggcgcccctagatgGTGTGGTGGCAATACGATCA
Left Primer PCR2 GCGCGAATTCTGGCGCCCCTAGATG(ETU)
Right Primer PCR1 cgcatgtagcctgccCGTAGTGCTCCTCCATGACA
Right Primer PCR2 GCGCTCTAGACGCATGTAGCCTGCC(XTS3)
Sequence Verification Primer ETU
Template genomic
Sequence GTGTGGTGGCAATACGATCACTGGTTCCATTGAACCTGTTTTTTTCCGACAGCAACATGTCCAAATGCGTCTCGAAGAAACCCCCTAGCCCGTAGTTAATGACCTGATAGAACTCCGTAGAGTTCATGTCCAGCCCAGTCGCATCTCCCAAGCGCTCGGTTATCTTCTTGGTCGTATCGTTGAAGTCACTGCTATACCAAACAGACTTTGAGGTGCGGTATGTGTCGACTTGTGTTTCGTCATCGGATGCATCTGGGTCAGTTGTGGCAGACGGCAACATGTGTTCCTTGCTCGAAGTTCTAATGAGAGTACTCTCCTTTTGGGAGATCATGTCATGGAGGAGCACTACG