100421
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SKU
KK-100421
| VDRC Id | 100421 |
|---|---|
| Library | KK |
| Status | available |
| Construct Id | 104274 |
| CG Number (r6.01) | CG5732 |
| FlyBase gene number | FBgn0038934 |
| Synonyms | Dm1 DmCG5732 DmGLD2 Gld2 |
| Inserted Chromosome | 2 |
| Viability | viable |
| Hairpin Length | 516 |
| ON Targets | 1 |
| OFF Targets | 0 |
| CAN Repeats | 3 |
| Insertion @ 40D | N |
| Insertion @ 30B | Y |
| Left Primer PCR1 | tggcgcccctagatgCCAGCTAGATTGGCGAGTTC |
| Left Primer PCR2 | GCGCGAATTCTGGCGCCCCTAGATG(ETU) |
| Right Primer PCR1 | taggtctagccccgcCTGGCCACAAAGATGGTTTT |
| Right Primer PCR2 | GCGCTCTAGATAGGTCTAGCCCCGC(XTS2) |
| Sequence Verification Primer | ETU |
| Template | genomic |
| Sequence | CCAGCTAGATTGGCGAGTTCGTCCGATGGCATTGACCGTGAAGCAGTGGGCCCAGTATCACAACATTAACAATGCCAAGAACATGACCATATCGAGCTACTCGCTCATGCTAATGGTCATTCATTTCCTGCAGGTTGGAGCAAGTCCACCTGTGCTTCCATGTCTGCACAACTTATATCCGGAGAAGTTTGGTCTCCTGCAGCCCAACGATTTCGGTTATGTGGACATGAACGAGGTGATGGCTCCGTATCAGTCGGATAACAGCCAAACTCTAGGTGATTTGCTGTTGAGCTTCCTACACTACTACAGTGTATTTGATTACGGAAAGTATGCCATCTCGATACGGGTGGGTGGTGTCCTGCCCATAGAGGTGTGCCGGGCAGCCACTGCGCCCAAAAACGATATACACCAGTGGAACGAGCTGTGCATCGAAGAGCCATTCGATCAGACGAATACAGCTCGATCGGTGTATGATACGGATACATTTGAACGCATCAAAACCATCTTTGTGGCCAG |