100327
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SKU
KK-100327
| VDRC Id | 100327 |
|---|---|
| Library | KK |
| Status | available |
| Comment | hemilethal |
| Construct Id | 105928 |
| CG Number (r6.01) | CG2666 |
| FlyBase gene number | FBgn0001311 FBgn0013580 FBgn0037319 |
| Synonyms | Blimp CS-1 CS-1/kkv ChSB Chitin Synthase B Chitin synthase 1 DmCS-1 DmeChSB Kkv Krotzkopf verkehrt blimp chitin synthase-1 kkv krotzkopf verkehrt krotzkopf-verkehrt l(3)82Fh short of breath |
| Inserted Chromosome | 2 |
| Viability | sterile |
| Hairpin Length | 346 |
| ON Targets | 1 |
| OFF Targets | 0 |
| CAN Repeats | 2 |
| Insertion @ 40D | Y |
| Insertion @ 30B | Y |
| Left Primer PCR1 | tggcgcccctagatgAAACAACTTCTCGGTGGTGG |
| Left Primer PCR2 | GCGCGAATTCTGGCGCCCCTAGATG(ETU) |
| Right Primer PCR1 | taggtctagccccgcGCTTCTACAGATTTTCGGCG |
| Right Primer PCR2 | GCGCTCTAGATAGGTCTAGCCCCGC(XTS2) |
| Sequence Verification Primer | ETU |
| Template | genomic |
| Sequence | AAACAACTTCTCGGTGGTGGCCAGACGCTCGCACTTCGGAGTCCAGATGTGCAGTGCTATCCAGGTCTGACTGAGGAGCCAAAGGATCCAGGCCCACGCCATTTGCTCGGTGACAAAGTTGTTGAACCGGAAGTTCGAGGGGCTCGTGAAGAACAGATAGTCCGGAATGGTGTCATGGAAGAAGCAGGGATCGTCGATCCGGATTCCACAGGCTGCGATCAGGAACGTAACAGAGAGCGGAACAGTTAGACTGACGGGAAACGCGTAGCTGAATCCCTGGATGAGGATCTTGCAGGCGAACTTTCCGAAGATGTAGCACAAGTAGGCGCCGAAAATCTGTAGAAGC |