100300

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SKU
KK-100300
More Information
VDRC Id 100300
Library KK
Status available
Construct Id 107637
CG Number (r6.01) CG7578
FlyBase gene number FBgn0027855
FBgn0028538
Synonyms BG:DS00797.7
BIG1
Sec71
Sec71 ortholog (S. cerevisiae)
sec71
Inserted Chromosome 2
Viability viable
Hairpin Length 551
ON Targets 1
OFF Targets 0
CAN Repeats 3
Insertion @ 30B Y
Left Primer PCR1 tggcgcccctagatgTGAAGGATCAGGAATCGGTC
Left Primer PCR2 GCGCGAATTCTGGCGCCCCTAGATG(ETU)
Right Primer PCR1 cgacgcccgctgataAGCACAAATACGTGTCAGCG
Right Primer PCR2 GCGCTCTAGACGACGCCCGCTGATA(XTS1)
Sequence Verification Primer ETU
Template genomic
Sequence TGAAGGATCAGGAATCGGTCGGTGAGCCAGAGCCAACACTTAATGGAAACGACTACTCCTCGCACTCGGATCACGACAGTGTGGAGCTGCACAGCGAAAACGATGCGGTTGTAACGGCTAAGTTTACGCACATCCTGCAGAAAGATGCTTTTCTCGTGTTCCGGGCACTGTGCAAGCTATCGATGAAGCCTTTGCCGGATGGACATCCAGATCCGAAATCGCACGAGCTGCGTTCCAAGGTGCTGTCATTGCATCTGCTGCTGCTCATCCTCCAGAATGCCGGGCCCGTCTTCCGCTCCAACGAGATGTTCATCATGGCCATTAAGCAGTACCTGTGCGTGGCCTTGTCAAACAACGGAGTCAGTCTGGTGCCGGAGGTCTTCGAGCTGTCGCTTTCAATCTTCGTTGCCCTACTCTCGAACTTCAAGGTGCATCTTAAGCGGCAGATAGAGGTGTTCTTCAAGGAAATCTTCCTAAACATTCTTGAGGCGAACTCAAGCAGCTTCGAGCACAAATGGATGGTAATCCAAGCGCTGACACGTATTTGTGCT