100217
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SKU
KK-100217
| VDRC Id | 100217 |
|---|---|
| Library | KK |
| Status | available |
| Construct Id | 107137 |
| CG Number (r6.01) | CG7918 |
| FlyBase gene number | FBgn0037546 FBgn0046498 FBgn0046499 |
| Synonyms | B-type mAChR Dm-mAChR-B anon-WO0170980.91 anon-WO0170980.92 mAChR B mAChR-B mAcR-CG7918 muscarinic Acetylcholine Receptor, B-type |
| Inserted Chromosome | 2 |
| Viability | viable |
| Hairpin Length | 415 |
| ON Targets | 1 |
| OFF Targets | 0 |
| CAN Repeats | 3 |
| Insertion @ 40D | N |
| Insertion @ 30B | Y |
| Left Primer PCR1 | tggcgcccctagatgACGTGCTCAAGGGTTATTGG |
| Left Primer PCR2 | GCGCGAATTCTGGCGCCCCTAGATG(ETU) |
| Right Primer PCR1 | cgacgcccgctgataTCACTGCGCTTTTGCATATC |
| Right Primer PCR2 | GCGCTCTAGACGACGCCCGCTGATA(XTS1) |
| Sequence Verification Primer | ETU |
| Template | genomic |
| Sequence | ACGTGCTCAAGGGTTATTGGGACCTGGGTCCGATGCTCTGCGATCTCTGGCTTTCCGTCGACTATACTGTGTGTCTGGTGTCCCAGTACACGGTGCTGTTGATCACGATAGATCGCTACTGCTCTGTGAAGATTGCCGCCAAGTACCGGAGTTGGAGGACGAGAACCAGAGTGATATATATGGTCACAATCACTTGGATCATTCCCGCTCTGCTCTTCTTCATCTCGATTTTTGGATGGGAGCACTTCACCGGCAAGCGGGATCTACTTCCTGGCCAGTGCGCCGTTCAGTTTCTCAAGGATCCGATCTTTAATACGGCCCTAATCATTGGCTACTATTGGACCACACTAATTGTGCTTTTTGTCTTATATGCCGGGATCTACAAAACAGCCTATGATATGCAAAAGCGCAGTGA |